浅沼幹人
Professor　

脳神経機構学（旧解剖学第三） 教授（2014.7.1〜）

Department of Medical Neurobiology

  

★を＠にかえてください

専門 :

神経薬理化学 (Neurochemical Pharmacology)
神経保護・修復の分子生物学 (Molecular Biology of Neuroprotection and Neurorestoration)

研究テーマ

１． パーキンソン病などの神経変性疾患モデルの病態解析とその保護・修復（Study on pathophysiology and neuroprotection for neurodegenerative diseases）
       新しい治療法の開発（Development of novel therapeutic strategies)

２． 神経疾患の治療標的としてのアストロサイトの役割の解明（Study on role of astrocyte as a neurotherapeutic target）

３． 乱用薬物（覚醒剤，違法ドラッグ）の神経毒性と防御（Neurotoxicity of drugs abused (methamphetamine, illegal drugs)）

研究キーワード

パーキンソン病 (Parkinson's disease)
ドパミン (dopamine)
キノン体 (quinone)
酸化ストレス (oxidative stress)
乱用薬物 (drug abused)
グリア細胞 (glia)

主な研究業績

Asanuma, M., Miyazaki, I., Murakami, S., Diaz-Corrales, F.J. and Ogawa, N.: Striatal astrocytes act as a reservoir for L-DOPA. PLoS ONE, 9(9): e106362, 2014. doi:10.1371/journal.pone.0106362.

Murakami, S., Miyazaki, I., Sogawa, N.*, Miyoshi, K.*, Asanuma, M.:Neuroprotective effects of metallothionein against rotenone-induced myenteric neurodegeneration in Parkinsonian mice.  Neurotox. Res., 26: 285-98, 2014. DOI10.1007/s12640-014-9480-1.

Miyazaki, I., Asanuma, M., Murakami, S., Takeshima, M., Torigoe, N., Kitamura, Y. and Miyoshi, K.: Targeting 5-HT1A receptors in astrocytes to protect dopaminergic neurons in parkinsonian models. Neurobiol. Dis.,  59: 244-256, 2013．

Asanuma, M., Miyazaki, I., Diaz-Corrales, F.J.*, Higashi, Y.*, Namba, M.* and Ogawa, N.: Transplantation of melanocytes obtained from the skin ameliorates apomorphine-induced abnormal behavior in rodent hemi-parkinsonian models.  PLoS ONE, 8(6): e65983, 2013. doi:10.1371/journal.pone.0065983.

Diaz-Corrales, F.J., Miyazaki, I., Asanuma, M., Ruano, D.* and Rios, R.M.*: Centrosomal aggregates and Golgi fragmentation disrupt vesicular trafficking of DAT. Neurobiol. Aging, 33:2462-2477, 2012.

Asanuma, M., Miyazaki, I., Kikkawa, Y., Kimoto, N., Takeshima, M., Murakami, S.,　Miyoshi, K.: Cyclooxygenase-independent neuroprotective effects of aspirin against dopamine quinone-induced neurotoxicity. Neurochem. Res., 37: 1944-1951, 2012.

Miyazaki, I., Asanuma, M., Kikkawa, Y., Takeshima, M., Murakami, S., Miyoshi, K., Sogawa, N. and Kita, T.: Astrocyte-derived metallothionein protects dopaminergic neurons from dopamine quinone toxicity. Glia, 59: 435-451, 2011.

Ogawa, D., Asanuma, M., Miyazaki, I., Tachibana, H.*, Wada, J.*, Sogawa, N.*, Sugaya, T.*, Kitamura, S.*, Maeshima, Y.*, Shikata, K.* and Makino, F.*:High glucose increases metallothionein expression in renal proximal tubular epithelial cells. Exp. Diabetes Res., i 2011: 534872, 2011.

Asanuma, M., Miyazaki, I., Diaz-Corrales, F.J., Kimoto, N., Kikkawa, Y., Takeshima, M., Miyoshi, K. and Murata, M.*
Neuroprotective effects of zonisamide target astrocyte. 　Ann. Neurol., 67:239-249, 2010.

Tsuji, T.,Asanuma, M., Miyazaki, I., Miyoshi, K. and Ogawa, N.
Reduction of nuclear peroxisome proliferator-activated receptor γ expression in methamphetamine-induced neurotoxicity and neuroprotective effects of ibuprofen. Neurochem. Res., 34:764-774, 2009.

Miyoshi, K., Kasahara, K., Miyazaki, I., Shimizu, S.*, Taniguchi, M.*, Matsuzaki, S.*, Tohyama, M.*, Asanuma, M.
Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.
FASEB J., 23: 3289-3297, 2009.

Miyoshi K, Kasahara K, Miyazaki I, Asanuma M.: Lithium treatment elongates primary cilia in the mouse brain and in cultured cells. Biochem. Biophys. Res. Commun., 388 757-762, 2009.

Asanuma, M., Miyazaki, I., Diaz-Corrales, F.J., Miyoshi, K., Ogawa, N. and Murata, M.
Preventing effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone formation.
Neurosci. Res., 60: 106-113, 2008.

Hozumi, H., Asanuma, M., Miyazaki, I., Fukuoka, S., Kikkawa, Y., Kimoto, N., Kitamura, Y., Sendo, T., Kita, T. and Gomita, Y.: Protective effects of interferon-gamma against methamphetamine-induced neurotoxicity. Toxicol. Lett., 177: 123-129, 2008.

Shimizu, M., Miyazaki, I., Higashi, Y., Eslava-Alva, M.J., Diaz-Corrales, F.J., Asanuma, M. and Ogawa, N.: Specific induction of PAG608 in cranial and spinal motor neurons of L-DOPA-treated parkinsonian rats. Neurosci. Res., 60: 355-363, 2008.

Miyazaki, I., Asanuma, M., Hozumi, H., Miyoshi, K. and Sogawa, N.: Protective effects of metallothionein against dopamine quinone-induced dopaminergic neurotoxicity. FEBS. Lett., 581: 5003-5008, 2007.

Asanuma, M. and Miyazaki, I.: Common anti-inflammatory drugs are potentially therapeutic for Parkinson's disease? Exp. Neurol., 206: 172-178, 2007.

Miyoshi, K., Asanuma, M., Miyazaki, I., Matsuzaki, S., Tohyama, M. and Ogawa, N.: Characterization of pericentrin isoforms in vivo. Biochem. Biophys. Res. Commun., 351: 745-749, 2006.

Miyazaki, I., Asanuma, M., Diaz-Corrales, F.J., Fukuda, M., Kitaichi, K., Miyoshi, K. and Ogawa, N.: Methamphetamine-induced dopaminergic neurotoxicity is regulated by quinone formation-related molecules. FASEB J., 20: 571-573, 2006 (published online January 10, 2006).

Miyazaki, I., Asanuma, M., Diaz-Corrales, F.J., Miyoshi, K. and Ogawa, N.: Dopamine agonist pergolide prevents levodopa-induced quinoprotein formation in parkinsonian striatum and shows quenching effects on dopamine-semiquinone generated in vitro. Clin. Neuropharmacol., 28: 155-160, 2005.

Diaz-Corrales, F.J., Asanuma, M., Miyazaki, I., Miyoshi, K. and Ogawa, N.: Rotenone induces aggregation of gamma-tubulin protein and subsequent disorganization of the centrosome: Relevance to formation of inclusion bodies and neurodegeneration. Neuroscience, 133: 117-135, 2005.

Miyazaki, I., Asanuma, M., Diaz-Corrales, F.J., Miyoshi, K. and Ogawa, N.: Direct evidence for expression of dopamine receptors in astrocytes from basal ganglia. Brain Res., 1029: 120-123, 2004.

Higashi, Y., Asanuma, M., Miyazaki, I., Hattori, N., Mizuno, Y. and Ogawa, N.: Parkin attenuates manganese-induced dopaminergic cell death. J. Neurochem., 89: 1490-1497, 2004.

Miyoshi, K., Asanuma, M., Miyazaki, I., Diaz-Corrales, F. J., Katayama, T., Tohyama, M. and Ogawa, N.: DISC1 localizes to the centrosome by binding to kendrin. Biochem. Biophys. Res. Commun., 317: 1195-1199, 2004.

Asanuma, M., Miyazaki, I. and Ogawa, N.: Dopamine- or L-DOPA-induced neurotoxicity: the role of dopamine quinone formation and tyrosinase in a model of Parkinson's disease. Neurotox. Res., 5: 165-176, 2003.

Haque, M.E., Asanuma, M., Higashi, Y., Miyazaki, I., Tanaka, K. and Ogawa, N.: Apoptosis-inducing neurotoxicity of dopamine and its metabolites via reactive quinone generation in neuroblastoma cells. Biochim. Biophys. Acta, 1619: 39-52, 2003.

Higashi, Y., Asanuma, M., Miyazaki, I., Haque, M.E., Fujita, N., Tanaka, K. and Ogawa, N.: The p53-activated gene, PAG608, requires a zinc finger domain for nuclear localization and oxidative stress-induced apoptosis. J. Biol. Chem., 277: 42224-42232, 2002.

Asanuma, M., Nishibayashi-Asanuma, S., Miyazaki, I., Kohno, M. and Ogawa, N.: Neuroprotective effects of non-steroidal anti-inflammatory drugs by direct scavenging of nitric oxide radicals. J. Neurochem., 76: 1895-1904, 2001.

Higashi, Y., Asanuma, M., Miyazaki, I. and Ogawa, N.: Inhibition of tyrosinase reduces cell viability in catecholaminergic neuronal cells. J. Neurochem., 75: 1771-1774, 2000.

Asanuma, M., Hayashi, T., Ordonez, S.V., Ogawa, N. and Cadet, J.L.: Direct interactions of methamphetamine with the nucleus. Mol. Brain Res., 80: 237-243, 2000.

Iwata-Ichikawa, E., Kondo, Y., Miyazaki, I., Asanuma, M. and Ogawa, N.: Glial cells protect neurons against oxidative stress via transcriptional up-regulation of the glutathione synthesis. J. Neurochem., 72: 2334-2344, 1999.

Asanuma, M. and Cadet, J.L.: Methamphetamine-induced increase in striatal NF-kB DNA-binding activity is attenuated in superoxide dismutase transgenic mice. Mol. Brain Res., 60: 305-309, 1998.

Gomez-Vargas, M., Nishibayashi-Asanuma, S., Asanuma, M., Kondo, Y., Iwata, E. and Ogawa, N.: Pergolide scavenges both hydroxyl and nitric oxide free radicals in vitro and inhibits lipid peroxidation in different regions of the rat brain. Barin Res., 790: 202-208, 1998.

Asanuma, M., Hirata, H. and Cadet, J.L.: Attenuation of 6-hydroxydopamine-induced dopaminergic nigrostriatal lesions in superoxide dismutase transgenic mice. Neuroscience, 85: 907-917, 1998.

Asahara, H., Fujisawa, K., Kobata, T., Hasunuma, T., Maeda, T., Asanuma, M., Ogawa, N., Inoue, H., Sumida, T. and Nishioka, K.: Direct evidence of high DNA-binding activity of transcription factor AP-1 in the synovium with rheumatoid arthritis. Arthritis Rheum., 40: 912-918, 1997.

Nishibayashi, S., Asanuma, M., Kohno, M., Gomez-Vargas, M. and Ogawa, N.: Scavenging effects of dopamine agonists on nitric oxide radicals. J. Neurochem., 67: 2208-2211, 1996.

Asanuma, M., Nishibayashi, S., Kondo, Y., Iwata, E., Tsuda, M. and Ogawa, N.: Effects of single cyclosporin A pretreatment on pentylenetetrazol-induced convulsion and on TRE-binding activity in the rat brain. Mol. Brain Res., 33: 29-36, 1995.

Asanuma, M., Ogawa, N., Nishibayashi, S., Kawai, M., Kondo, Y. and Iwata, E.: Protective effects of pergolide on dopamine levels in the 6-hydroxydopamine-lesioned mouse brain. Arch. Int. Pharmacodyn. Ther., 329: 221-230, 1995.

Kondo, Y., Ogawa, N., Asanuma, M., Ota, Z. and Mori, A.: Regional differences in late onset iron deposition, ferritin, transferrin, astrocyte proliferation and microglial activation after transient forebrain ischemia in rat brain. J. Cereb. Blood Flow Metab., 15: 216-226, 1995.

Ogawa, N., Tanaka, K., Asanuma, M., Kawai, M., Masumizu, T., Kohno, M. and Mori, A.: Bromocriptine protects mice against 6-hydroxydopamine and scavenges hydroxyl free radicals in vitro. Brain Res., 657: 207-213, 1994.

Ogawa, N., Asanuma, M., Kondo, Y., Kawada, Y. and Mori, A.: Differential effects of chronic L-DOPA treatment on lipid peroxidation in the mouse brain with or without pretreatment with 6-hydroxydopamine. Neurosci. Lett., 171: 55-58, 1994.

Asanuma, M., Ogawa, N., Haba, K., Hirata, H. and Mori, A.: Effects of chronic catecholamine depletions on muscarinic M1-receptor and its mRNA in rat brain. J. Neurol. Sci., 110: 205-214, 1992.

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