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生理学実習(心臓のランゲンドルフ灌流) Physiology Practice

実験データ(「右クリック→名前を付けてリンク先を保存」してください)

01班 02班 03班 04班 05班

06班 07班 08班 09班 10班

 

1班の人は、WebClassの「資料」にランゲンドルフ灌流の実験ビデオがあるので、そちらを参照してください。

データは上記03班のものを使用してください。

 

レポートの書き方の相談は、随時受け付けています。

基礎研究棟6F 第4研究室・高橋まで気軽に相談してください。

 

trophy

よいレポートの書き方

論文抄読会 Journal Club

毎週開催しています。

Sessions are held every week.


Journal articles

■17.08.24.-09.07.

Li, Y., Asfour, H. & Bursac, N. Age-dependent functional crosstalk between cardiac fibroblasts and cardiomyocytes in a 3D engineered cardiac tissue. Acta Biomater 55, 120-130, doi:10.1016/j.actbio.2017.04.027 (2017).

■17.08.03.-08.10.

Ohno, Y. et al. Distinct iPS Cells Show Different Cardiac Differentiation Efficiency. Stem Cells Int 2013, 659739, doi:10.1155/2013/659739 (2013).

■17.07.13.-17.07.20.

Iribe, G. et al. Axial stretch of rat single ventricular cardiomyocytes causes an acute and transient increase in Ca2+ spark rate. Circ Res 104, 787-795, doi:CIRCRESAHA.108.193334 [pii] 10.1161/CIRCRESAHA.108.193334 (2009).

■17.06.22.-17.06.29.

Giacomelli, E. et al. Three-dimensional cardiac microtissues composed of cardiomyocytes and endothelial cells co-differentiated from human pluripotent stem cells. Development 144, 1008-1017, doi:10.1242/dev.143438 (2017).

■17.05.18.-17.06.08.

Ong, S. G. et al. Microfluidic Single-Cell Analysis of Transplanted Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes After Acute Myocardial Infarction. Circulation 132, 762-771, doi:10.1161/CIRCULATIONAHA.114.015231 (2015).

■17.04.27.-17.05.11.

Seta, H., Matsuura, K., Sekine, H., Yamazaki, K. & Shimizu, T. Tubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo. Sci Rep 7, 45499, doi:10.1038/srep45499 (2017).

■17.03.31.-17.04.13.

Ruan, J. L. et al. Mechanical Stress Conditioning and Electrical Stimulation Promote Contractility and Force Maturation of Induced Pluripotent Stem Cell-Derived Human Cardiac Tissue. Circulation 134, 1557-1567, doi:10.1161/CIRCULATIONAHA.114.014998 (2016).

■17.03.10.-17.03.16.

Rother J, Richter C, Turco L, et al. (2015) Crosstalk of cardiomyocytes and fibroblasts in co-cultures. Open Biol 5: 150038.

■17.02.16.-17.03.01.

Hof T, Salle L, Coulbault L, et al. (2016) TRPM4 non-selective cation channels influence action potentials in rabbit Purkinje fibres. J Physiol 594: 295-306.

■17.02.01.-17.02.09.

Huebsch N, Loskill P, Deveshwar N, et al. (2016) Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses. Sci Rep 6: 24726.

■17.01.19.-17.01.25.

Ou D, Wang Q, Huang Y, et al. (2016) Co-culture with neonatal cardiomyocytes enhances the proliferation of iPSC-derived cardiomyocytes via FAK/JNK signaling. BMC Dev Biol 16: 11.

■16.12.07.-17.01.12.

Tohyama S, Fujita J, Hishiki T, et al. (2016) Glutamine Oxidation Is Indispensable for Survival of Human Pluripotent Stem Cells. Cell Metab 23: 663-674.

■16.11.11.-16.12.02.

Liang J, Huang W, Cai W, Wang L, Guo L, Paul C, et al. (2016) Inhibition of microRNA-495 Enhances Therapeutic Angiogenesis of Human Induced Pluripotent Stem Cells. Stem Cells.

■16.10.07.-16.11.11.

Lahti AL, Kujala VJ, Chapman H, Koivisto AP, Pekkanen-Mattila M, Kerkela E, et al. (2012) Model for long QT syndrome type 2 using human iPS cells demonstrates arrhythmogenic characteristics in cell culture. Dis Model Mech 5(2): 220-30.

■16.09.23.-16.10.07.

Stevens KR, Kreutziger KL, Dupras SK, Korte FS, Regnier M, Muskheli V, et al. (2009) Physiological function and transplantation of scaffold-free and vascularized human cardiac muscle tissue. Proc Natl Acad Sci U S A 106(39):16568-73.

■16.09.12.-16.09.16.

Constantine, M., et al. (2016) "Heterologously-expressed and Liposome-reconstituted Human Transient Receptor Potential Melastatin 4 Channel (TRPM4) is a Functional Tetramer." Sci Rep 6: 19352.

■16.05.26.-16.07.14.

Sirabella D, Cimetta E, Vunjak-Novakovic G. (2015) "The state of the heart": Recent advances in engineering human cardiac tissue from pluripotent stem cells. Exp Biol Med 240(8):1008-1018

■15.09.03.

Bartolak-Suki E, Imsirovic J, Parameswaran H, Wellman TJ, Martinez N, et al. (2015) Fluctuation-driven mechanotransduction regulates mitochondrial-network structure and function. Nat Mater.

■15.05.20.-15.06.03.

Yamashiro K, Myokai F, Hiratsuka K, Yamamoto T, Senoo K, et al. (2007) Oligonucleotide array analysis of cyclic tension-responsive genes in human periodontal ligament fibroblasts. Int J Biochem Cell Biol 39: 910-921.

■15.05.15.

Hayakawa Y, Aoyama T, Yokoyama C, Okamoto C, Komaki H, et al. (2015) High salt intake damages the heart through activation of cardiac (pro) renin receptors even at an early stage of hypertension. PLoS One 10: e0120453.

■15.05.12.

Chouchani ET, Pell VR, Gaude E, Aksentijevic D, Sundier SY, et al. (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature 515: 431-435.

■15.04.15.-15.05.13.

Nagai Y, Yokoi H, Kaihara K and Naruse K (2012) The mechanical stimulation of cells in 3D culture within a self-assembling peptide hydrogel. Biomaterials 33: 1044-1051.

■15.01.23.

Zimmermann WH, Melnychenko I, Wasmeier G, Didie M, Naito H, et al. (2006) Engineered heart tissue grafts improve systolic and diastolic function in infarcted rat hearts. Nat Med 12: 452-458.

■14.12.17.

Shen JB, Yang R, Pappano A and Liang BT (2014) Cardiac P2X purinergic receptors as a new pathway for increasing Na+ entry in cardiac myocytes. Am J Physiol Heart Circ Physiol 307: H1469-1477.

■14.11.21.

Lundy SD, Zhu WZ, Regnier M and Laflamme MA (2013) Structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells. Stem cells and development 22: 1991-2002.

■14.11.07.-14.11.14.

Ubil E, Duan J, Pillai IC, Rosa-Garrido M, Wu Y, et al. (2014) Mesenchymal-endothelial transition contributes to cardiac neovascularization. Nature 514: 585-590.

■14.09.26.-14.10.03.

Makarewich CA, Zhang H, Davis J, Correll RN, Trappanese DM, et al. (2014) Transient receptor potential channels contribute to pathological structural and functional remodeling after myocardial infarction. Circulation research 115: 567-580.

■14.09.05.

Majkut, S., T. Idema, J. Swift, C. Krieger, A. Liu and D. E. Discher (2013). Heart-specific stiffening in early embryos parallels matrix and myosin expression to optimize beating. Curr Biol 23(23): 2434-2439.

■14.06.27.-14.07.25.

Shaw RM, Colecraft HM (2013) L-type calcium channel targeting and local signalling in cardiac myocytes. Cardiovasc Res 98: 177-186.

■14.04.25.-14.06.06.

Sonkusare SK, et al. (2012) Elementary Ca2+ signals through endothelial TRPV4 channels regulate vascular function. Science 336(6081): 597-601.

■14.03.14.-14.04.02.

Kinehara M, Kawamura S, Tateyama D, et al. (2013) Protein kinase C regulates human pluripotent stem cell self-renewal. PLoS One 8(1): e54122.

■14.02.21.

Tang L, Gamal El-Din TM, Payandeh J, et al. (2014) Structural basis for Ca2+ selectivity of a voltage-gated calcium channel. Nature 505: 56-61.

■14.02.14.

Obokata, H. et al. Stimulus-triggered fate conversion of somatic cells into pluripotency. Nature, 2014. 505 (7485), p. 641-647.

■14.01.10.-14.01.17.

Scimia, M.C. et al. APJ acts as a dual receptor in cardiac hypertrophy. Nature, 2012. 488 (7411), p. 394-398.

■13.12.20.-13.12.27.

Mathar I, Kecskes M, Van Der Mieren G, et al. Increased beta-Adrenergic Inotropy in Ventricular Myocardium from Trpm4-/- Mice. Circ Res, 2014. 114(2): p. 283-294.

■13.12.06.-13.12.13.

Narayan, N., et al. The NAD-dependent deacetylase SIRT2 is required for programmed necrosis. Nature, 2012. 492(7428): p. 199-204.

■13.10.24.-13.11.21.

Rahman, K.S., et al. Modeling the Conformational Changes Underlying Channel Opening in CFTR. Plos One, 2013. 8(9): p. e74574.

■13.10.11.-13.10.18.

Banjo T, et al. Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21. Nat Commun, 2013. 4: p.1978.

■13.08.16.-13.10.04.

Laflamme M.A and Murry C.E. Heart regeneration. Nature, 2011. 473(7347): p. 326-35.

■13.08.01.-13.08.09.

Brohawn, S.G., J. del Marmol, and R. MacKinnon, Crystal structure of the human K2P TRAAK, a lipid- and mechano-sensitive K+ ion channel. Science, 2012. 335(6067): p. 436-41.

■13.06.28.-13.07.18.

Chouchani, E.T., et al. Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Nat Med, 2013. 19(6): p. 753-9.

■13.06.21.-13.07.04.

Borgia, M.B., et al. Single-molecule fluorescence reveals sequence-specific misfolding in multidomain proteins. Nature, 2011. 474(7353): p. 662-5.

■13.05.

Yamada, S., et al. Protection conferred by myocardial ATP-sensitive K+ channels in pressure overload-induced congestive heart failure revealed in KCNJ11 Kir6.2-null mutant. J Physiol, 2006. 577(Pt 3): p. 1053-65.

 

Book chapters

■16.07.21-16.09.02.

History of electrophysiology and the patch clamp. in: Martina, M. and S. Taverna (2014). Patch-clamp methods and protocols. New York, Humana Press.

■12.07.01-14.09.30.

Normal physiology of the cardiovascular system. Hurst's the Heart

■13.06.03.-13.09.30.

Membrane structure. Molecular Biology of the Cell

■12.10.22.-13.06.xx.

Molecular and cellular mechanisms of myocardial ischemia-reperfusion injury. Hurst's the Heart

■12.07.02.-13.03.xx.

Ion channels and the electrical properties of membranes. Molecular Biology of the Cell

■11.xx

Planr patch clamping. Patch-clamp analysis

 

使用教科書 Textbooks

Patch-Clamp Analysis - Advanced Techniques 2nd edition. Edited by Wolfgang Walz

Molecular Biology of the Cell 5th edition

Hurst's the Heart13th edition

トップサイエンティストの英語を聴く!サーチュインに魅せられた研究者たち

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