Discrimination of glycoproteins via two-color laser-induced fluorescence detection
July 08, 2013
Researchers in the Okayama University Graduate School of Natural Science and Technology have developed a unique system for discrimination of glycoproteins.
The new system determines which protein has sugar-chains in a protein mixture sample by two-color laser induced fluorescence detection. The protein sample can be analyzed without employing an enzyme treatment beforehand.
The research will be published in the journal Electrophoresis.
( in press; doi:10.1002elps.201300149)
T. Kaneta and A. Tabara have developed the system for discrimination of glycoproteins via two-color laser-induced fluorescence detection coupled with postcolumn derivatization in capillary electrophoresis. A sample of protein mixture is incubated with dye and dye-labeled lectin, and separated by capillary electrophoresis.
450nm and 532nm laser beams are irradiated alternately to the separated sample, and the intensity of fluorescence at 450nm and 532nm emitted from the sample is recorded simultaneously. The dye binds to protein and is excited by the 450nm beam. The dye-labeled-lectin binds to sugar-chains and is excited by the 532nm beam; as a result, glycoprotein has both kinds of fluorescence at 450nm and 532nm.
Sugar chains are associated with the functions of protein such as antibodies and protein markers for cancer. Therefore, the technique for discrimination of glycoproteins is very important in the research fields of medicine, pharmaceuticals and biology. The new system could improve the quality control of antibody drugs and the screening of protein markers for cancer.
This study was funded by the Japan Society for the Promotion of Science (JSPS).
Contact Information:
Mototaka Senda, Ph.D.
US Representative
Intellectual Property Office, Organization for Research Promotion and Collaboration, Okayama University
Fremont, California USA
TEL: 1-510-797-0907
Email: takasenda@okayama-u.ac.jp
Takashi Kaneta, Ph.D.
Graduate School of Natural Science and Technology, Okayama University, Okayama Japan