Publications (January 1st, 2001 〜 )

 

90.    Hattori, T., Kubota, S., Yutani, Y.,Fujisawa,T.,Nakanishi, T.,Takahashi, K. and Takigawa, M.: Change incellularlocalization ofa rheumatoidarthritis-related antigen (RA-A47) withdownregulationuponstimulation byinflammatory cytokines in chondrocytes. J.Cell. Physiol.,186,268-281, 2001.

91.    Kubo, T., Arai , Y., Takahashi, K., Ikeda,T., Ohashi, S., Kitajima,I.,Mazda,O. and Takigawa, M., Imanishi, J. andHirasawa, Y.: Expressionoftransduced HSP70gene protects chondrocytes fromstress. J. Rheumatol., 28, 330-335, 2001.

92.    Ohashi, S., Kubo, T., Ikeda, T., Arai, Y.,Takahashi, K.,Hirasawa,Y.,Takigawa, M., Satoh, E., Imanishi, J. and Mazda,O.:Cationicpolymer-mediatedgenetic transduction into culturedhumanchondrosarcoma-derived HCS-2/8 cells. J. Orthop. Sci., 6, 75-81,2001.

93.    Sekine, T., Masuko- Hongo, K., Matui, T., Asahara,H.,Takigawa,M.,Nishioka, K. and Kato, T.: YKL-39, a humancartilage-relatedprotein, isrecognizedas a target antigen in patients withrheumatoid arthritis.Ann.Rheum. Dis., 60,49-54, 2001.

94.    Nakanishi, T., Yamaai, Y., Asano, M., Nawachi, K.,Suzuki,M.,Sugimoto, T.and Takigawa, M.: Overexpressions of connectivetissuegrowthfactor/hypertrophicchondrocyte specific geneproduct24(CTGF/Hcs24)decreasesbone density in adultmice and inducesdwarfism.Biochem. Biophys. Res.Commun., 281, 678-681, 2001.

95.    Boumediene, K., Takigawa, M. and Pujol,J-P.:Celldensity-dependentproliferative effects of transforming growth factor(TGF)-β1, β2, and β3inhumanchondrosarcomacells HCS-2/8 are associated with changes in the expressionofTGF-βreceptortype ・.CancerInvest.,19,485-495, 2001.

96.    Hirashima, Y., Kobayashi, H., Suzuki, M., Tanaka, Y.,Kanayama,N.,Fujie,M., Nishida, T., Takigawa, M. and Terao, T.:Characterizationofbindingproperties of uninary trypsin inhibitor (UTI) tocell-associatedbindingsiteson human chondrosarcoma cell line HCS-2/8. J. Biol.,Chem., 276,13650-6,2001.

97.    Eguchi, T., Kubota, S., Kondo, S., Shimo, T., Hattori,T.,Nakanishi,T.,Kuboki, T., Yatani, H. and Takigawa, M.: Regulatory mechanismofhumanconnectivetissue growth factor (ctgf/hcs24) gene expression inahumanchondrocytic cellline, HCS-2/8. J. Biochem., 130, 79-87, 2001.

98.    Tsuboi, Y., Nakanishi, T., Takano- Yamamoto, T.,Miyamoto,M.,Yamashiro,T. and Takigawa, M.: Mitogenetic effect ofneurotrophinsonperiodonatal ligamentcell line. J. Dent. Res., 80, 881-886,2001.

99.    Kubota, S., Eguchi, T., Shimo, T., Nishida, T., Hattori,T.,Kondo,S.,Nakanishi, T. and Takigawa, M.: Novel mode of processingandsecretionofconnective tissue growth factor/ecogenin (CTGF/Hcs24)inchondrocyticHCS-2/8cells. Bone, 29,155-161, 2001.

100.  Kubota, S.: The extinction program for homo sapiensandcloninghumans:Trinucleotide expansion as a one-way track toextinction.Med.Hypotheses, 56,296-301, 2001.

101.  Sakai,T., Kambe, F., Mitsuyama, H., Ishiguro, N.,Kurokouchi,K.,Takigawa, M., Iwata,H. and Seo, H.: Tumor necrosis factor α induces expression ofgenesfor matrix degradation inhumanchondrocyte-like HCS-2/8 cellsthroughactivation of NF-KB: Abrogation ofthetumor necrosis factor α effectbyproteasomeinhibitors. J. Bone Miner. Res., 16, 1272-1280,2001.

102.  Shimo, T., Nakanishi, T., Nishida, T., Asano, M., Sasaki,A.,Kanyama,M.,Kuboki, T., Matsumura, T. and Takigawa, M. : Involvement ofCTGF,ahypertrophicchondrocyte-specific gene product, in tumorangiogenesis.Oncology,61, 315-322,2001.

103.  Shimo, T., Kubota, S., Kondo, S., Nakanishi, T., Sasaki,A.,Mese,H.,Matsumura, T. and Takigawa, M.: Connectivetissue growth factorasamajorangiogenic agent that is induced by hypoxia in a human breast cancercellline.CancerLett., 174, 57-64, 2001.

104.  Yosimichi, G., Nakanishi, T., Nishida, T., Hattori,T.,Takano-Yamamoto,T. and Takigawa, M.: CTGF/Hcs24 induceschondrocytedifferentiationthrough ap38 mitogen-activated protein kinase(p38MAPK),andproliferationthrough ap44/42 MAPK/extracellular-signal regulatedkinase (ERK).Eur. J. Biochem.268,6058-6065, 2001.

105.  Kubota, S., Mukudai, Y., Hattori, T., Eguchi, T., Kondo,S.andTakigawa,M.:Cell-type-specific trans-activation of herpessimplexvirusthymidine kinasepromoter by the human T-cell leukemia virus Type・ Tax protein. DNAcellBiol.,20, 563-568, 2001.

106.  Yamashiro, T., Fukunaga, T., Kobashi, N., Kamioka,H.,Nakanishi,T.,Takigawa, M. and Takano-Yamamoto, T.: MechanicalstimulationinducesCTGFexpression in rat osteocytes. J. Dent. Res., 80, 461-465,2001.

107.  Oka, T., Yoshino, T., Hayashi, K., Ohara, N., Nakanishi,T.,Yamaai,Y.,Hiraki, A., Aoki-Sogawa, C., Kondo, E., Teramoto, N.,Takahashi,K.,Tsuchiyama,J. and Akagi, T.:Reducion of hematopoieticcell-specifictyroshinephosphataseShp-1 gene expression in natural killer celllymphoma andbarioustypes oflymphomas/leukemias:Combination analysis with cDNAexpressionarrayand tissuemicyoarra., American Journal of Pathology, 159,1495-1505,2001.

108.  Pan, L-H., Yamauchi, M., Uzuki, M., Nakanishi, T.,Takigawa,M.,Inoue, H.and Sawai, T.:Type ・ albeolarepithelial cells and interstitialfibroblastsexpressconnective tissue growthfactor in IPF. Eur. Respir. J. 17,1220-1227,2001

109.  Suzuki, M., Kobayashi,H., Fujie, M., Nishida, T., Takigawa,M.,Kanayama,N. and Terao, T.:Kunitz-typeprotease inhibitor bikunindisruptsphorbolester-induced oligomerization of CD44variant isoformscontainingepitope v9 andsubsequently suppresses expression ofurokinase-typeplasminogenactivator inhuman chondrosarcoma cells. The Journal ofBiologicalChemistry,277, 8022-8032,2002

110.  Sironen, R. K.,Karjalainen, H. M., Torronen, K., Elo,M.A.,Kaarniranta, K., Takigawa, M.,Helminen, H. J.and Lammi, M. J.:Highpressureeffects on cellular expressionprofile and mRNA stability. AcDNAarrayanalysis. Biorheology, 39, 111-117, 2002.

111.  Kondo, S., Kubota, S.,Shimo, T., Nishida, T., Yoshimichi,G.,Eguchi,T., Sugahara, T. and Takigawa, M.:Connective tissue growthfactorincreased byhypoxia may initiate angiogenesis incollaborationwithmatrixmetalloproteinases. Carcinogenesis, 23, 769-776, 2002.

112.  Sakata, M.,Masuko-Hongo, K., Tsuruha, J., Sekine, T.,Nakamura,H.,Takigawa, M., Nishioka,K. and Kato, T.: YKL-39, ahumancartilage-relatedprotein, induces arthritis inmice. Clin Exp Rheumatol.,20,343-50, 2002

113.  Tsuruha, J.,Masuko-Hongo, K., Kato, T., Sakata, M.,Nakamura,H.,Sekine, T., Takigawa, M. andNishioka, K.: Autoimmunity againstYKL-39, ahumancartilage-deribed protein inpatients with osteoarthritis. J.Rheum.,29,1459-66, 2002.

114.  Nishida, T., Kubota,S., Nakanishi, T., Kuboki, T., Yosimichi,G.,Kondo,S. and Takigawa, M.:CTGF/Hcs24, a hypertrophic chondrocytespecificgeneproduct, stimulates theproliferation and expression of thecartilagephenotypebut not hypertrophy orcalcification or articular cartilageinculture. J Cell.Physiol., 192, 55-63,2002.

115.  Eguchi, T., Kubota, S.,Kondo, S., Kuboki, T., Yatani,H.andTakigawa, M.: A novel cis-element thatenhances connective tissuegrowthfactorgene expression in chondorocytic cells.Biochem. Bophys. Res.Commun.,395,445-451, 2002.

116.  Fang, J., Kubota, S.and Pomerantz, R. J.: Atrans-dominantnegativeHIV-1 Rev with intact domains ofNLS/NOS and NES. AIDSRes.& Hum.Retro.,18, 705-709, 2002.

117.  Sironen, R. K.,Karjalainen, H. M., Elo, M.A., Kaarniranta,K.,Torronen,K., Takigawa, M.,Helminen, H. J. and Lammi, M. J.: cDNA arrayrevealsmechanosensitive genes inchondrocytic cells underhydrostaticpressure. Biochim BiophysActa 19,45-54, 2002.

118.  Suzuki, M., Kobayashi,H., Fujie., M, Nishida, T., Takigawa,M.,Kanayama,N. and Terao, T.:Kunitz-typeprotease inhibitor bikunindisruptsphorbolester-induced oligomerization of CD44variant isoformscontainingepitope v9 and subsequently suppresses expression ofurokinase-typeplasminogenactivator inhuman chondrosarcoma cells. J. Biol.Chem., 277, 8022-8032,2002.

119.  Kobayashi, H., Suzuki,M., Kanayama, N., Nishida, T., Takigawa,M. and Terao, T.: Suppression ofurokinase receptor expression by bikuninisassociatedwith inhibition of upstreamtargets ofextracellularsignal-regulatedkinase-dependent cascade. Eur. J.Biochem., 269,16,3945-3957, 2002.

120.  Suzuki, M., Kobayashi,H., Kanayama, N., Nishida, T., Takigawa,M. and Terao, T.: CD44 stimulation byfragmented hyaluronic acidinducesupregulationand tyrosine phosphorylation ofc-Met receptor protein inhumanchondrosarcomacells. Biochim. Biophys.Acta.,1591, 37-44, 2002.

121.  Yamamoto, Y., FurukawaK., Ueyama, K., Nakanishi, T., Takigawa,M.andHarata, S.: Possible roles ofCTGF/Hcs24 in the initiation anddevelopmentofossification longitudinalligament. Spine. 27, 1852-1857, 2002.

122.  Nakata, E., Nakanishi,T., Kawai, A., Asaumi, K., Yamaai,T., Asano,M., Nishida, T., Mitani, S., Inoue,H. and Takigawa, M.:Expressionofconnective tissue growth factor/hypertrophicchondorocyte-specificgeneproduct24 (CTGF/Hcs24) during fracture healing. Bone,31, 441-447, 2002.

123.  Nawachi, K., Inoue, M.,Kubota, S., Nishida, T.,Yosimichi,G.,Nakanishi, T., Kanyama, M., Kuboki, T.,Yatani, H., Yamaai, T.andTakigawa,M.: Tyrosine kinase-type receptor ErbB4 in chondrocytes:interactionwithconnectivetissue growth factor and distribution in cartilage.FEBS Lett.,528,109-13,2002.

124.  Inoue, T., Okada, H.,Kobayashi, T., Watanabe, Y., Kikuta,T.,Kanno,Y., Takigawa, M. and Suzuki, H.:TGF-beta1 and HGFcoordinatelyfacilitatecollagen turnover in subepithelialmesenchyme. Biochem.Biophys. Res.Commun.,297, 255-260, 2002.

125.  Kobayashi, H., Suzuki,M., Kanayama, N., Nishida, T., Takigawa,M.andTerao, T.: CD44 stimulation byfragmented hyaluronic acidinducesupregulation ofurokinase-type plasminogenactivator and its receptorandsubsequentlyfacilitates invasion of humanchondrosarcoma cells. Int.J.Cancer, 102, 379-389,2002.

126.  Yosimichi, G., Kubota,S., Hattori, T., Nishida, T.,NawachiK,Nakanishi, T., Kamada, M,Takano-Yamamoto, T and Takigawa,M.:CTGF/Hcs24interacts with the cytoskeletoalprotein actin inchondrocytes.  Biochem.Bophys. Res.Commun., 299,755-761,2002.

127.  Shimizu,H.,Nishitani, Y., Yamada, T., Nishida, T., Takigawa, M.andYoshiyama,M.:Development of dentin regeneration therapy: expression oftype Icollagenandalkaline phosphatase induced by CTGF in human cultureddental pulp.J.HardTissue Biology, 11, 62-67, 2002.

128.  Karjalainen, H. M.,Sironen, R.K., Elo, M. A., Kaarniranta,K.,Takigawa,M., Helminen, H. J. andLammi, M. J.: Gene expression profilesinchondrosarcomacells subjected to cyclicstretching and hydrostatic pressure.AcDNA arraystudy. Biorheology, 40, 93-100,2003.

129.  Inoue, T., Okada, H.,Kobayashi, T., Watanabe, Y., Kanno, Y., Kopp,J.B., Nishida, T., Takigawa, M.,Ueno, M., Nakamura, T. and Suzuki,H.:Hepatocytegrowth factor counteractstransforming growth factor-beta1,throughattenuationof connective tissue growthfactor induction, and preventsrenalfibrogenesis in5/6 nephrectomized mice.FASEB J., 17, 268-270, 2003.

130.  Mukudai, Y., Kubota,S., and Takigawa, M.:Conservedrepressiveregulation of connective tissue growthfactor/hypertrophicchondrocyte-specificgene 24 (ctgf/hcs24) enabled bydifferentelements andfactors among vertebratespecies. Biol. Chem., 384, 1-9,2003.

131.  Brigstock DR,Goldschmeding R, Katsube KI,LamSC,Lau LF, Lyons K, Naus C, Perbal B, Riser B,Takigawa M, Yeger H.:Proposalfor aunified CCN nomenclature. Mol Pathol. 56(2),127-8, 2003.

132.  Moritani, N. H.,Kubota, S., Kondo, S., Nishida, T., Kawaki,H.,Sugahara,T. and Takigawa, M:Suppressive effect of overexpressedconnectivetissue growthfactor on tumor cellgrowth in a human oral squamouscellcarcinoma-derived cellline. Cancer Lett.,192(2), 205-14, 2003.

133.  Nishida, T., Kubota S.,Fukunaga, T., Kondo, S., Yosimichi,G., Nakanishi, T., Takano-Yamamoto, T. andTakigawa, M.: CTGF/Hcs24, hypertrophicchondrocyte-specific gene product,interacts withperlecan inregulating theproliferation and differentiation ofchondrocytes. J.Cell.Physiol., 196(2),265-75, 2003.

134.  Moritani, N. H.,Kubota, S., Eguchi, T., Fukunaga, T., Yamashiro,T., Takano-Yamamoto, T.,Tahara, H., Ohyama, K., Sugahara, T. and Takigawa,M.: Interaction of AP-1 andthe ctgf gene: a possible driverofchondrocytehypertrophy in growth cartilage.J. Bone Miner. Metab.,21(4),205-10, 2003.

135.  Kuboki, T., Kanyama,M., Nakanishi, T., Akiyama, K., Nawachi,K.,Yatani, H., Yamashita, K.,Takano-Yamamoto, T. and Takigawa,M.: Cbfa1/Runx2gene expression in thetemporomandibular and knee jointarticularchondrocytesin vivo. Archs. of OralBiol., 48(7), 519-25, 2003.

136.  Hattori, T., Kawaki,H., Kubota, S., Yutani, Y., deCrombrugghe,B.,von der Mark, K., and Takigawa,M.: Downregulation ofarheumatoidarthritis-related antigen (RA-A47) by ra-a47antisenseoligonucleotidesinducesinflammatory factors in chondrocytes. J. Cell.Physiol.,197, 94-102,2003.

137.  Fujisawa,T.,Kuboki, T., Kasai, T., Sonoyama,W.,Kojima,S.,Uehara, J., Komori, C., Yatani, H., Hattori, T. and Takigawa,M.:Arepetitivesteady mouth opening induced osteoarthritis-likelesioninrabbittemporomandibular joint. J. Dent. Res., 82, 731-735, 2003.

138.  Kanyama, M., Kuboki,T., Akiyama, K., Nawachi, K., Miyauchi,F.M.,Yatani, H., Kubota, S., Nakanishi,T. and Takigawa, M.:ConnectiveTissueGrowth Factor Expressed in Rat AlveolarBone Regeneration SitesafterToothExtraction. Arch. Oral Biol., 48, 723-730,2003.

139.  Kawaki, H., Kubota, S.,Minaro, M., Moritani, N. H.,Hattori,T.,Hanagata, H., Kubota, M., Miyauchi, A.,Nakanishi, T. and Takigawa,M.:Novelenzyme-linked immunosorbent assay systems forthe quantitativeanalysesofconnective tissue growth factor (CTGF/Hcs24/CCN2):DetectionofHTLV-1tax-induced CTGF from a human carcinoma cell line. DNA CellBiol.,22,641-648,2003.

140.  Fukunaga, T.,Yamashiro, T., Oya, S., Takeshita, N., Takigawa,M.andTakano-Yamamoto, T.:Connective tissue growth factor mRNAexpressionpattern incartilages isassociated with their type I collagenexpression.Bone, 33(6),911-8, 2003.

141.  Kubota, S., Moritani,N. H., Kawaki, H., Mimura, H., Minato,M.andTakigawa, M.: Transcriptional inductionof connective tissuegrowthfactor/hypertrophic chondrocyte specific 24(CTGF/Hcs24) gene bydexamethasoneinhuman chondrocytic cells. Bone, 33: 694-702,2003.

142.  Yokoi, H., Mukoyama,M., Nagae, T., Mori, K., Suganami, T.,Sawai,K.,Yoshioka, T., Koshikawa, M.,Nishida, T., Takigawa, M., Sugawara, A.andNakao,K.: Reduction in connectivetissue growth factor byantisensetreatmentameliorates renal tubulointerstitialfibrosis. J. Am. Soc.Nephrol.,15,1430-40, 2004.

143.  Minato, M., Kubota, S.,Kawaki, H., Nishida, T.,Miyauchi,A.,Hanagata, H., Kubota, M., Nakanishi, T.,Yamamoto-Takano, T.andTakigawa,M.: Module-specific antibodies against humanconnective tissuegrowthfactor:Utility for structural and functional analysis ofthe factor asrelatedtochondrocytes. J. Biochem. 135 347-354. 2004.

144.  Nishida, T., Kubota,S., Kojima, S., Kuboki, T., Nakao,K.,Kushibiki,T., Tabata, Y. and Takigawa. M.Regeneration of defects inarticularcartilage inrat knee joints by CCN2(connective tissue growthfactor). J. BoneMiner. Res.,19, 1308-1319, 2004.

145.  Kadota,H.,Nakanishi,T.,Asaumi, K., Yamaai, T., Nakata, E., Mitani, S., Aoki, K.,Aiga,A., Inoue, HandTakigawa, M.: Expression of ConnectiveTissueGrowthFactor/HypertrophicChondrocyte-Specific Gene Product24(CTGF/Hcs24/CCN2)During DistractionOsteogenesis. J. Bone Miner. Metab.,22(4),293-302, 2004.

146.  Jung, y., Jong, J.,Ryoo, H.,Kim,H.,Kim, Y., Park, E., Si, H., Kim, S., Takigawa, M., Lee, B.,Park, R,Kim,I., Choi,J.: Gene expression profile ofhumanchondrocyte HCS 2/8cellline by EST sequencing analysis. Gene, 330,85-92,2004.

147.  Kaji T, Yamamoto C,Oh-I M,NishidaT,Takigawa M.: Differential regulation of biglycan anddecorinsynthesisbyconnective tissue growth factor in cultured vascularendothelialcells. Biochem Biophys Res Commun., 322, 22-28, 2004

148.  Hattori, T., von derMark, K., Kawaki, H., Yutani, Y.,Kubota,S.,Nakanishi, T., Eberspeacher, H., deCrombrugghe, B. andTakigawa,M.Downregulation of rheumatoidarthritis-relatedantigenRA-A47(=HSP47/Colligin-2) in chondrocytic cell linesinducesapoptosisandcell-surface expression of RA-A47 in association with CD9.J.Cell.Physiol.,197(1), 94-102, 2004.

149.  Gebhard, S., Pöschl,E., Riemer, S., Bauer, E., Hattori, T.,Eberspaecher, H., Zhang, Z., Lefebvre,V., de Crombrugghe, B. and von der Mark,K. A.: Highly Conserved Enhancer inMammalian Type X Collagen Genes Drives HighLevels of Tissue-Specific Expressionin Hypertrophic Cartilage in vitro and invivo. Matrix Biol., 23(5), 309-322,2004.

150.  Fushimi, K., Nakashima,S.,Takigawa, M. and Shimizu,K.: Implication of prostaglandin E2 in TNF-a-inducedrelease of m-calpain fromHCS-2/8 chondrocytes: Inhibition of m-calpain releaseby NSAIDs Osteoarthritisand Cartilage, 12(11), 895-903, 2004.

151.  Omoto, S., Nishida, K.,Shibahara,M., Nishida, T., Doi, T., Asahara, H., Nakanishi, T., Inoue, H. andTakigawa,M.: Expression and localization of Connective tissue growthfactor(CTGF/Hcs24/CCN2) by chondrocytes in osteoarthritic cartilage.Osteoarthirtisand Cartilage, 12(10), 771-778, 2004.

152.  Kubota, S., Kawata,K.,Yanagita, T., Doi, H., Kitoh, T. and Takigawa, M.: Abundant retentionreleaseof connective tissue growth factor (CTGF/CCN2) by platelets. J.Biochem. 136,279-82, 2004.

153.  Saas J., Lindauer K.,Bau B.,Takigawa M., and Aigner T.: Molecular phenotyping of HCS-2/8 cells as aninvitro model of human chondrocytes. Osteoarthritis Cartilage 12(11), 924-34,2004.

154.  Fang, J., Kubota, S.,Yang,B., Zhou, N., Zhang, H., Godbout, R. and Pomerantz, R.J.: A DEAD boxproteinfacilitates HIV-1 Replication as a cellular co-factor of Rev. Virology.330,471-480, 2004. 

155.  Hattori, T., von derMark,K., Kawaki, H., Yutani, Y., Kubota, S., Nakanishi, T., Eberspeacher, H.,deCrombrugghe, B. and Takigawa, M. Downregulation of rheumatoidarthritis-relatedantigen RA-A47 (=HSP47/Colligin-2) in chondrocytic cell linesinduces apoptosisand cell-surface expression of RA-A47 in association with CD9.J. Cell.Physiol., 202, 191-204, 2005.

156.  Mukudai, Y., Kubota,S., Eguchi,T., Kondo, S., Nakao, K. and Takigawa, M.: Regulation of chickenccn2 gene by interaction between RNA cis-elementand trans-factor duringdifferentiation of chondrocytes. J. Biol. Chem., 280,3166-3177, 2005.

157.  Okada, H., Kikuta,T.,Kobayashi, T., Inoue, T., Kanno, Y., Takigawa, M., Sugaya, T., Kopp, J.B.andSuzuki, H: Connective tissue growth factor expressed in tubular epitheliumplaysa pivotal role in renal fibrogenesis. J. Am. Soc. Nephrol. 16(1),133-143, 2005.

158.  Chujo, S., Shirasaki,F.,Kawara, S., Inagaki, Y., Kinbara, T., Inaoki, M., Takigawa, M. andTakehara,K.: Connective tissue growth factor causes persistent proa2(I)collagengene expression induced by transforming growth factor-b in a mousefibrosis model.J. Cell. Physiol. 203(2), 447-456, 2005.

159.  Gebauer, M., Saas, J.,Haag,J., Dietzb, U., Takigawa, M., Aigner, T.: Repression of anti-proliferativefactorTob1 in osteoarthritic cartilage. Arthritis Research Therapy, 7, R274-R284,2005.

160.  Chrysis, D., Zaman, F.,Chagin, AS., Takigawa, M., Sävendahl, L.: Dexamethasoneinduces apoptosisin proliferative chondrocytes through activation of caspasesand suppression ofthe Akt-(PI3K) signalling pathway. Endocrinology, 46(3),1391-1397, 2005.

161.  Nakao, K., Kubota, S.,Doi,H., Eguchi, T., Oka, M., Fujisawa,T., Nishida, T. and Takigawa, M.:Collaborative action of M-CSF and CTGF/CCN2 inarticular chondrocytes:possibleregenerative roles in articular cartilagemetabolism. Bone, 36, 884-892, 2005.

162.  Yamaai, T., Nakanishi,T.,Asano, M., Nawachi, K., Yosimichi, G., Ohyama, K., Komoari, T., Sugimoto,T.and Takigawa, M.: Gene expression of connective tissue growthfactor(CTGF/CCN2) in calcifying tissues of normal and cbfa1-null mutant in latestageof mouse embryonic development. J. Bone Miner. Metab., 23, 280-288, 2005.

163.  Moritani,NH., Kubota,S., Sugahara, S. and Takigawa, M.: Comparable response of ccn1 with ccn2 genesupon arthritis: An invitroevaluation with a human chondrocytic cell linestimulated by a set of cytokines.Cell Communication and Signaling, 3(1), 6,2005.

164.  Tsuchiya, T., Osanai,T.,Ogose, A., Tamura, G., Chano, T., Kaneko, Y., Ishikawa, A., Orui, H., Wada,T.,Ikeda, T., Namba, M., Takigawa, M., Kawashima, H., Hotta, T., Tsuchiya,A.,Ogino, T. and Motoyama, T.: Methylation status of EXT1 and EXT2 promotersandtwo mutations of EXT2 in chondrosarcoma. Cancer Genet Cytogenet. 158(2),148-155,2005.

165.  Kubota, S., Mukudai, Y.,Moritani,N.H., Nakao, K, Kawata, K. and KTakigawa, M.: Transcriptionalrepression by the cis-acting element ofstructure-anchored repression (CAESAR)of human ctgf/ccn2 mRNA. FEBS Lett, 579,3751-3758, 2005.

166.  Kobayashi, T., Inoue,T.,Okada, H., Kikuta, T., Kanno, Y., Nishida, T., Takigawa, M. Sugaya, T.andSuzuki, H.: Connective tissue growth factor mediates the profibrotic effectsoftransforming growth factor-beta produced by tubular epithelial cellsinresponse to high glucose. Clin Exp Nephrol. 9(2), 114-121, 2005.

167.  Saas, J., Gebauer, M.,Jacobi,C., Haag, J., Takigawa, M., Aigner, T.: The human chondrosarcoma HCS-2/8cellline is responsive to BMP-7, but not to IL-1beta.  Front. Biosci., 10, 2027-2035, 2005.

168.  Asano, M., Kubota,S.,Nakanishi, T., Nishida, T., Yamaai, T., Yosimichi, G., Ohyama, K.,Sugimoto,T., Murayama, Y. and Takigawa, M.: Effect of connective tissue growthfactor(CCN2/CTGF) on proliferation and differentiation of mouseperiodontalligament-derived cells. Cell Commun Signal. 2005 Oct 5;3(1):11（Epub Journal）

169.  Hong MeiPiao, Koheiyamauchi, Li-Hua Pan, Toshihide Nakadate, Harumasa Ito, TakashiMouri, HitoshiKobayashi, Takashi Sawai, Tohru Nakanishi, Masaharu Takigawa and Hiroshi Inoue:Increased Levels of CTGF mRNA Expression in aMurine Model of Allergic AirwayInflammation. Allergology International, 54,107-115, 2005.

170.  Maeda,T., Jikko, A.,Abe, M., Yokohama-Tamaki, T., Akiyama, H., Furukawa, S.,Takigawa, M. andWakisaka, S.: Cartducin, a Paralog of acrp30/adiponectin, is inducedduringchondrogenic differentiation and promotes proliferation of chondrogenicprecursorsand chondrocytes. J. Cell. Physiol., 206(2), 537-544, 2005.

171.  Kondo, S., Kubota,S.,Mukudai, Y., Moritani, N., Nishida, T., Matsushita, H.,Matsumoto, S.,Sugahara, T. and Takigawa, M.: Hypoxic regulation of stabilityof connectivetissue growth factor/CCN2 mRNA by 3’-untranslated region interactingwith acellular protein in human chondrosarcoma cells. Oncogene, 25(7):1099-1110,2006.

172.  Kondo, S., Tanaka,N.,Kubota, S., Mukudai, Y., Yosimichi, G., Sugahara T., and Takigawa, M.:Novelangiogenic inhibitor DN-9693 that inhibits post-transcriptional inductionofconnective tissue growth factor (CTGF/CCN2) by vascular endothelialgrowthfactor (VEGF) in human endothelial cells. Molecular Cancer Therapeutics5, 129-137,2006.

173.  Yosimichi, G., Kubota,S.,Nishida, T., Kondo, S., Yanagita, T., Nakao, K., Takano-Yamamoto, T.andTakigawa, M.: Roles of PKC, PI3K and JNK in multiple transduction ofCCN2/CTGFsignals in chondrocytes. Bone, 38, 853-863, 2006.

174.  Hoshijima M., Hattori,MT., Inoue,M.,Araki, D, Hanagata, H. Miyauchi, A., and Takigawa, M. CT domainof CCN2/CTGF directlyinteracts with fibronectin and enhances cell adhesion ofchondrocytes integrin a5b1. FEBS Lett. 580(5), 1376-1382, 2006.

175.  Okada,H., Kikuta, T.,Inoue, T., Kanno, Y., Ban, S., Sugaya, T., Takigawa, M., andSuzuki, H.:Dexamethasone induced connective tissue growth factor expression inrenaltugbular epithelial cells in a mouse strain-specific manner. Am. J.Pathol.168(2), 737-743, 2006.

176.  Chagin,A. S., Chrysis,D., Takigawa, M., Ritzen, E. M., and SavendahI, L. Locallyproduced estrogenpromotes fetal rat metatarsal bone growth; an effect mediatedthrough increasedchondrocyte proliferation and decreased apoptosis. J.Endocrinol., 188(2),193-203, 2006.

177.  Kubota,S., andTakigawa, M. CCN family genes in the development and differentiationofcartilage tissues. Clin. Calcium, 16(3), 486-492, 2006, Japanese.

178.  Kawata, K., Eguchi,T.,Kubota, S., Kawaki, H., Oka, M., Minagi,S., and Takigawa, M. : Possible roleof LRP1, a CCN2 receptor, in chondrocytes.Biochem Biophys Res Commun., 345(2):552-559, 2006.

179.  Hattori, T.,Eberspaecher,H., Lu, J., Zhang, R., Nishida, T., Kahyo, T., Yasuda, H., deCrombrugghe, B. :Interactions between PIAS proteins and SOX9 results in anincrease in thecellular concentrations of SOX9. J. Biol. Chem.,281(20):14417-14428, 2006.

180.  Kubota, S., Kawaki,H.,Kondo, S., Yosimichi, G., Minato, M., Nishida, T., Hanagata, H., Miyauchi,A.,Takigawa, M. Multiple activation of mitogen-activated protein kinasesbypurified independent CCN2 modules in vascular endothelial cellsandchondrocytes in cultue. Biochimie, in press, 2006.

181.  Shimo, T., Kubota, S.,Eguchi, T., Ibaragi, S., Isowa, S., Yoshioka,N., Takigawa, M., Sasaki, A. Pathogenicrole of connective tissue growth factor(CCN2/CTGF) in osteolytic metastasis ofbreast cancer. J. Bone Miner. Res.,21:1045-1059, 2006. 

182.  Schmidl M., Adam N.,Surmann-Schmitt C., Hattori T., Stock M., Dietz U., de Crombrugghe B., PöschlE., and von der Mark K. Twisted gastrulation modulates bone morphogeneticprotein-induced collagen II and X expression in chondrocytes in vitro and invivo. J. Biol. Chem. 281(42): 31790-31800, 2006.

183.  Kondo, S., Kubota, S.,Sugahara, T. and Takigawa, M. Expression and regulation of an anti-sense RNAtranscript from human connective tissue growth factor gene in human cells.  Asian J. Oral Mallilofa. Surg., 18: 172-179,2006.

184.  Aiga A., Asaumi K., LeeY. J., Kadota H., Mitani S., Ozaki T., Takigawa M.  Expression of neurotrophins and their receptorstropomyosin-related kinases (Trk) under tension-stress during distractionosteogenesis.. Acta Med Okayama., 60(5):267-277, 2006.

185.  Mori M., Nakajima M.,Mikami Y., Seki S., Takigawa M., Kubo T., Ikegawa S. Transcriptional regulationof the cartilage intermediate layer protein (CILP) gene.  Biochem. Biophys. Res. Commun. 341, 121-127,2006.

186.  Gebhard S., Hattori T.,Bauer E., Bosl M.R., Schlund B., Poschl E., Adam N., de Crombrugghe B., von derMark K.　BAC constructs in transgenic reporter mouse linescontrol efficient and specific LacZ expression in hypertrophic chondrocytesunder the complete Col10a1 promoter. Histochem Cell Biol., 127(2): 183-194,2007.

187.  Zaman F,Menendez-Benito V, Eriksson E, Chagin AS, Takigawa M, Fadeel B, Dantuma NP,Chrysis D and Savendahl L Proteasome inhibition up-regulates p53 andapoptosis-inducing factor in chondrocytes causing severe growth retardation inmice. Cancer Res. 67(20): 10078-10086, 2007.

188.  Hirano Y, Ishiguro N,Sokabe M, Takigawa M and Naruse K Effects of tensile and compressive strains onresponse of a chondrocytic cell line embedded in type I collagen gel. J.Biotechnol. 133(2): 245-252, 2007.

189.  Fushimi K., NakashimaS.,You F., Takigawa M., Shimizu K. Prostaglandin E(2) downregulatesTNF-alpha-induced production of matrix metalloproteinase-1 in HCS-2/8chondrocytes by inhibiting Raf-1/MEK/ERK cascade through EP4 prostanoidreceptor activation. J. Cell Biochem. 100(3): 783-793, 2007.

190.  Hattori T. Takigawa M.Autoantigen and molecular chaperone in rheumatoid arthritis--their roles inmetabolism of chondrocytes]  ClinCalcium. 16(9): 133-136, 2006, Japanese.

191.  Chagin A. S., KarimianE., Zaman F., Takigawa M., Chrysis D. and Savendahl L. Tamoxifen inducespermanent growth arrest through selective induction of apoptosis in growthplate chondrocytes in cultured rat metatarsal bones. Bone, 40(5), 1415-1424,2007.

192.  Yanagita T., Kubota S.,Kawaki H., Kawata K., Kondo S., Takano-Yamamoto T., Tanaka S., Takigawa M.Expression and physiological role of CCN4/Wnt-induced secreted protein 1 mRNAsplicing variants in chondrocytes. FEBS J. 274(7): 1655-1665, 2007.

193.  Ono M., Kubota S.,Fujisawa T., Sonoyama W., Kawaki H., Akiyama K., Ohima M., Nishida T., YoshidaY., Suzuki K., Takigawa M., Kuboki T. Promotion of attachment of human bonemarrow stromal cells by CCN2. Biochem Biophys Res Commun. 357(1): 20-25, 2007.

194.  Koitabashi N., Arai M.,Kogure S., Niwano K., Watanabe A., Aoki Y., Maeno T., Nishida T., Kubota S.,Takigawa M., Kurabayashi M. Increased connective tissue growth factor relativeto brain natriuretic Peptide as a determinant of myocardial fibrosis.Hypertension. 49(5): 1120-1127, 2007.

195.  Eguchi T., Kubota S.,Kawata K., Mukudai Y., Ohgawara T., Miyazono K., Nakao K., Kondo S., TakigawaM. Different transcriptional strategies for ccn2/ctgf gene induction betweenhuman chondrocytic and breast cancer cell lines. Biochimie. 89(3): 278-288,2007.

196.  Nishida T., Kawaki H.,Baxter R.M., DeYoung A., Takigawa M., Lyons K.M. CCN2 (Connective tissue growthfactor) is essential for extracellular matrix production and integrin signalingin chondrocytes. J Cell Commun Signal, 1, 45-58, 2007.

197.  Miyamato Y., MabushiA., Shi D., Kubo T., Takatori Y., Saito S., Fujioka M., Sudo A., Uchida A.,Yamamoto S., Ozaki K., Takigawa M., Tanaka T., Nakamura Y., Jiang Q., IkegawaS. A functional polymorphism in the 5’ UTR of GDF5 is associated withsusceptibility to osteoarthritis.  Nat.Genet. 39, 529-533, 2007.

198.  Oka M., Kubota S.,Kondo S., Eguchi T., Kuroda C., Kawata K., Minagi S., Takigawa M. Geneexpression and distribution of connective tissue growth factor (CCN2/CTGF)during secondary ossification center formation. J. Histochem. Cytochem., 55:1245-1255, 2007.

199.  Ono M., Kubota S.,Fujisawa T., Sonoyama W., Kawaki H., Akiyama K., Oshima M., Nishida T., YoshidaY., Suzuki K., Takigawa M., Kuboki T. Promotion of hydroxyapatite-associated,stem cell-based bone regeneration by CCN2. Cell Transplantation, 17(1-2),231-240, 2008.

200.  Matsumoto Y., MotokiT., Kubota S., Takigawa M., Tsubouchi H., Gohda E.  Inhibition of tumor-stromal interaction through HGF-Met signalingby valproic acid.  Biochem. Biophys.Res. Commun., 366(1):110-116, 2008.

201.  Doi H., Nishida K.,Yorimitsu M., Komiyama T., Kadota Y., Tetsunaga T., Yoshida A., Kubota S.,Takigawa M., Ozaki T. Interleukin-4 downregulates the cyclic tensilestress-induced matrix metalloproteinases-13 and cathepsin B expression by ratnormal chondrocytes.  Acta MedicaOkayama, 62(2), 119-126, 2008.

202.  Eguchi T, Kubota S,Kawata K, Mukudai Y, Uehara J, Ohgawara T, Ibaragi S, Sasaki A, Kuboki T,Takigawa M. Novel transcription factor-like function of human MMP3 regulatingCTGF/CCN2 gene.  Mol. Cell. Biol., 28:2391-2413, 2008.

203.  Koitabashi N., Arai M.,Niwano K., Watanabe A., Endoh M., Suguta M., Yokoyama T., Tada H., Toyama T.,Adachi H., Naito S., Oshima S., Nishida T., Kubota S., Takigawa M., KurabayashiM.  Plasma connective tissue growthfactor is a novel potential biomarker of cardiac dysfunction in patients withchronic heart failure. Eur. J. Heart Failure, 10(4): 373-379, 2008.

204.  Fujisawa T., HattoriT., Ono M., Uehara J., Kubota S., Kuboki T., Takigawa M. CCN family 2 /Connective tissue growth factor (CCN2/CTGF) stimulates proliferation anddifferentiation of auricular chondrocytes. Osteoarthritis and Cartilage, 16(7),787-795, 2008.

205.  Kawaki H., Kubota S.,Suzuki A., Yamada T., Matsumura T., Mandai, T., Yao, M., Maeda T., Lyons K.M.,Takigawa M. Functional requirement of CCN2 for intramembranous bone formationin embryonic mice.  Biochem. Biophys.Res. Commun., 366(2):450-456, 2008.

206.  Kawaki H., Kubota S.,Suzuki A., Lazar, N., Yamada, T., Matsumura, T., Ohgawara, T., Maeda, T.,Perbal, B. Lyons, K. M., Takigawa M. Cooperative regulation of chondrocyte differentiationby CCN2 and CCN3 shown by a comprehensive analysis of the CCN family proteinsin cartilage. J. Bone Miner. Res. 23(11): 1751-1764, 2008.

207.  Nishida T., Maeda A.,Kubota S., Takigawa M. Role of mechanical-stress inducible proteinHcs24/CTGF/CCN2 in cartilage growth and regeneration: -Mechanical stressinduces expression of Hcs24/CTGF/CCN2 in human chondrocytic cell line, HCS-2/8,rabbit hyaline costal chondrocytes and meniscus cells- Biorheology, 45(3-4),289-299, 2008.

208.  Sun G., Haginoya K., WuY., Chiba Y., Nakanishi T., Onuma A., Sato Y., Takigawa M., Iinuma K., TsuchiyaS. Connective tissue growth factor is overexpressed in muscles of humanmuscular dystrophy. J Neurol Sci. 267(1-2), 48-56, 2008.

209.  Kikuchi T., Kubota S.,Asaumi K., Kawaki H., Nishida T., Kawata K., Mitani S., Tabata Y, Ozaki T.,Takigawa M. Promotion of bone regeneration by CCN2 incorporated into gelatinhydrogel. Tissue Engineering Part A 14(6), 1089-1098, 2008.

210.  Hattori, T., Coustry,F., Stephens, S., Eberspaecher, H., Takigawa, M., Yasuda, H., de Crombrugghe,B. Transcriptional regulation of chondrogenesis by coactivator Tip60 viachromatin association with Sox9 and Sox5. Nucleic Acids Res. 36(9): 3011-3024,2008.

211.  Motoki, T., Sugiura,Y., Matsumoto, Y., Tsuji, T., Kubota, S., Takigawa, M., Gohda, E. Induction ofhepatocyte growth factor expression by maleic acid in human fibroblasts throughMAPK activation. J. Cell Biochem. 104(4): 1465-1476, 2008.

212.  Kuroda, C., Kubota, S.,Kawata, K., Aoyama, E., Sumiyoshi, K., Inoue, M., Minagi, S., Takigawa, M.Distribution, gene expression, and functional role of EphA4 duringossification. Biochem. Biophys. Res. Commun. 374(1): 22-27, 2008.

213.  Mukudai, Y., Kubota,S., Kawaki, H., Kondo, S. Eguchi, T., Sumiyoshi, K., Ohgawara, T., Shimo, T., Takigawa,M.  Post-transcriptional regulation ofchicken ccn2 gene expression by nucleophosmin/B23 during chondrocytedifferentiation. Mol. Cell Biol. 28(19): 6134-6147, 2008.

214.  Shimo, T. Kubota, S.,Goda, T., Yoshihama, Y., Kurio, N., Nishida, T., Ng, P. S., Endo, K., Takigawa,M. Sasaki, A. Clinical significance and pathogenic function of connectivetissue growth factor (CTGF/CCN2) in osteolytic mandibular squamous cellcarcinoma. Anticancer Res. 28 (4C): 2343-2348, 2008.

215.  Gebhard S., Hattori T.,Bauer E.,  Schlund B., Bösl　MR., deCrombrugghe B., von der Mark K. Specific expression of Cre recombinase inhypertrophic cartilage under the control of a BAC- Col10a1 promoter. MatrixBiol. 27(8): 693-699, 2008.

216.  Takeuchi, H., Kubota,S., Murakashi, E., Fukada, T., Hashimoto, S., Takigawa, M. and Numabe, Y.:Effect of TGF-β1 on CCN2/CTGF in normal human gingival fibroblasts andperiodontal ligament cells. J. periodontal. Res., 44: 161-169, 2009. 

217.  Nishida, T. Kondo, S.,Maeda, A., Kubota, S., Lyons, K. M., Takigawa, M. CCN family 2/ connectivetissue growth factor (CCN2/CTGF) regulates the expression of Vegf throughHif-1a expression in a chondrocytic cell line, HCS-2/8, under hypoxiccondition. Bone 44 (1): 24-31, 2009.

218.  Maeda, A., Nishida, T.,Aoyama, E., Kubota, S., Lyons, K. M., Kuboki, T., Takigawa, M. CCN family2/connective tissue growth factor modulates BMP signaling as a signalconductor, which action regulayes the proliferation and differentiation ofchondrocytes. J. Biochem. 145(2): 207-216, 2009.

219.  Ohgawara, T., Kubota,S., Kawaki, H., Kondo, S., Eguchi, T., Kurio, N., Aoyama, E., Sasaki, A.Takigawa, M. Regulation of chondrocytic phenotype by micro RNA 18a: Involvementof Ccn2/Ctgf as a major target gene. FEBS Lett. 583: 1006-1010, 2009.

220.  Aoyama E, Hattori T, HoshijimaM, Araki D, Nishida T, Kubota S, Takigawa M.: N-terminal domains of CCN protein2/connective tissue growth factor bind to aggrecan.  Biochem. J., 420:413-420,2009.

221.  Sakai Y, Balam TA, Kuroda S, Tamamura N, Fukunaga T, Takigawa M, Takano-Yamamoto T.:CTGF and apoptosis in mouse osteocytes induced by tooth movement.   J Dent Res., 88(4):345-50, 2009.

222.  Hikichi Y, Yoshimura K, Takigawa M. All-trans retinoic acid-induced ADAM28 degrades proteoglycans in human chondrocytes.   Biochem Biophys Res Commun. 386(2): 294-299, 2009.

223.  Hattori, T., Mueller, C., Gebhard, S., Bauer, E., Pausch, F., Schlund, B., Boesl, M., Hess, A., Surmann-Schmitt, C., von der Mark, H., de Crombrugghe, B., von der Mark. K. Sox9 is a major negative regulator of cartilage vascularisation, bone marrow formation, and endochondral ossification.  Development, 137, 901-911, 2010.

224.  Kawata K, Kubota S, Eguchi T, Moritani NM, Shimo T, Kondo, S, Nishida T, Minagi S, Takigawa M. Role of the low-density lipoprotein-1 in regulation of chondrocyte differentiation.  J. Cell. Physiol., 222:138-48, 2010.

225.  Sumiyoshi K, Kubota S, Furuta RA, Tasui K, Aoyama E, Kawaki H, Kawata K, Ohgawara T, Yamashiro T, Takigawa M. Thrombopoietic-mesenchymal interaction that may facilitate both endochondral ossification and pletelet maturation via CCN2.  J. Cell Commun. Signal., 4, 5-14, 2010.

226.  Lindahl E, Nyman U, Zaman F, Palmberg C, Cascante A, Shafqat J, Takigawa M, Savendahl L, Jornvall H, Joseph B. : Proinsulin C-peptide regulates ribosomal RNA expression.  J. Biol. Chem., 285(5): 3462-3469, 2010.

227.  Takeuchi H, Kubota S, Murakashi E, Zhou Y, Endo K, Ng PS, Takigawa M, Numabe Y.: Nicotine-induced CCN2: from smoking to periodontal fibrosis.  J. Dent. Res., 89(1): 34-9, 2010.

228.  Kawaki, H., Kubota, S., Aoyama, E., Fujita, N., Hanagata, H., Miyauchi, A., Nakai, K., Takigawa, M. Design and utility of CCN2 anchor peptide aptamers.  Biochimie, 92, 1010-1015, 2010.

229.  Sumiyoshi K, Kubota S, Ohgawara T, Kawata K, Nishida T, Shimo T, Yamashiro T, Takigawa M. Identification of miR-1 as a micro RNA that supports late stage differentiation of growth cartilage cells. Biochem. Biophys.  Res. Commun., 402, 286-290, 2010.

230.  Mukudai, Y., Kubota, S., Eguchi, T., Sumiyoshi, K., Janune, D., Kondo, S., Shintani, S. and Takigawa, M. : A coading RNA segment that enhances the ribosomal revruitment of chicken Ccn1 mRNA.  J Cell Biochem. 111, 1607-1618, 2010.

231.  Coustry, F., Oh, C., Hattori, T., Maity, S., de Crombrugghe, B. and Yasuda, H.: The dimerization domain of Sox9 is required for transcription activation of a chondrocyte-specific chromatin DNA template.  Nucleic Acids Res. 38, 6018-6028, 2010.