原 著
90) Hattori, T., Kubota,S.,Yutani, Y., Fujisawa, T., Nakanishi,T., Takahashi,
K. and Takigawa, M.: Changein cellular localization of arheumatoid arthritis-related
antigen (RA-A47)with downregulation uponstimulation by inflammatory cytokines
in chondrocytes.J. Cell. Physiol., 186,268-281, 2001.
91) Kubo, T., Arai ,Y.,Takahashi, K., Ikeda, T., Ohashi, S., Kitajima,
I., Mazda, O. and Takigawa, M.,Imanishi, J. andHirasawa, Y.: Expression
of transduced HSP70 gene protectschondrocytes fromstress. J. Rheumatol.,
28, 330-335, 2001.
92) Ohashi, S., Kubo, T.,Ikeda,T., Arai, Y., Takahashi, K., Hirasawa, Y., Takigawa,
M., Satoh, E.,Imanishi,J. and Mazda, O.: Cationic polymer-mediated genetic
transduction intoculturedhuman chondrosarcoma-derived HCS-2/8 cells. J.
Orthop. Sci., 6,75-81,2001.
93) Sekine, T., Masuko- Hongo, K.,Matui,T.,
Asahara, H., Takigawa, M., Nishioka, K. and Kato, T.: YKL-39, ahumancartilage-related
protein, is recognized as a target antigen in patientswithrheumatoid arthritis.
Ann. Rheum. Dis., 60, 49-54, 2001.
94) Nakanishi, T., Yamaai,Y.,Asano, M.,
Nawachi, K., Suzuki, M., Sugimoto, T. and Takigawa,M.:Overexpressions of
connective tissue growth factor/hypertrophicchondrocytespecific gene product
24(CTGF/Hcs24)decreases bone density in adultmice andinduces dwarfism. Biochem.
Biophys. Res. Commun., 281, 678-681, 2001.
95) Boumediene, K.,Takigawa, M.and Pujol, J-P.: Cell density-dependent proliferative
effects oftransforminggrowth factor (TGF)-β1, β2, and β3 in humanchondrosarcoma
cells HCS-2/8 are associated with changesin the expression ofTGF-β receptor
type ・. Cancer Invest., 19, 485-495, 2001.
96) Hirashima, Y., Kobayashi,H.,Suzuki, M.,
Tanaka, Y., Kanayama, N., Fujie, M., Nishida, T., Takigawa, M.andTerao, T.:
Characterization of binding properties of uninary trypsininhibitor(UTI) to
cell-associated binding sites on human chondrosarcoma celllineHCS-2/8. J.
Biol., Chem., 276, 13650-6, 2001.
97) Eguchi, T., Kubota, S.,Kondo,S., Shimo,
T., Hattori, T., Nakanishi, T., Kuboki, T., Yatani, H. andTakigawa,M.:
Regulatory mechanism of human connective tissue growth factor(ctgf/hcs24)gene
expression in a human chondrocytic cell line, HCS-2/8. J.Biochem., 130,79-87,
2001.
98) Tsuboi, Y., Nakanishi,T.,Takano-Yamamoto,
T., Miyamoto, M., Yamashiro, T. and Takigawa, M.:Mitogeneticeffect of
neurotrophins on periodonatal ligament cell line. J. Dent.Res., 80,881-886,
2001.（口腔生化・分子歯科学分野）
99) Kubota, S., Eguchi, T.,Shimo,T., Nishida,
T., Hattori, T., Kondo, S., Nakanishi, T. and Takigawa, M.:Novelmode of
processing and secretion of connective tissue growthfactor/ecogenin(CTGF/Hcs24)
in chondrocytic HCS-2/8 cells. Bone, 29,155-161,2001.
100) Kubota, S.: Theextinctionprogram for homo
sapiens and cloning humans: Trinucleotide expansionas aone-way track to
extinction. Med. Hypotheses, 56, 296-301, 2001.
101) Sakai, T., Kambe,F.,Mitsuyama, H., Ishiguro, N., Kurokouchi, K., Takigawa,
M., Iwata, H. andSeo,H.: Tumor necrosis factor α inducesexpression of genes
for matrix degradation in humanchondrocyte-like HCS-2/8cells through activation
of NF-KB: Abrogation of thetumor necrosis factor α effect by proteasome
inhibitors. J. Bone Miner. Res.,16,1272-1280, 2001.
102) Shimo, T., Nakanishi,T.,Nishida, T.,
Asano, M., Sasaki, A., Kanyama, M., Kuboki, T., Matsumura, T.andTakigawa, M.:
Involvement of CTGF, a hypertrophic chondrocyte-specific geneproduct,in tumor
angiogenesis. Oncology, 61, 315-322, 2001.
103) Shimo, T., Kubota, S.,Kondo,S., Nakanishi,
T., Sasaki, A., Mese, H., Matsumura, T. and Takigawa,M.:Connectivetissue growth
factor as a major angiogenic agent that is inducedbyhypoxia in a human breast
cancer cell line. Cancer Lett., 174, 57-64, 2001.
104) Yosimichi, G., Nakanishi,T.,Nishida, T.,
Hattori, T., Takano- Yamamoto, T. and Takigawa, M.:CTGF/Hcs24induces
chondrocyte differentiation through a p38 mitogen-activatedproteinkinase
(p38MAPK),and proliferation through a p44/42MAPK/extracellular-signalregulated
kinase (ERK). Eur. J. Biochem. 268,6058-6065, 2001.
105) Kubota, S.,Mukudai, Y.,Hattori, T., Eguchi, T., Kondo, S. and Takigawa,M.:Cell-type-specifictrans-activation
of herpes simplex virus thymidine kinasepromoter by the humanT-cell leukemia
virus Type・ Tax protein. DNA cell Biol., 20, 563-568, 2001.
106) Yamashiro, T., Fukunaga,T.,Kobashi, N.,
Kamioka, H., Nakanishi, T., Takigawa, M. and Takano-Yamamoto,T.:Mechanical
stimulation induces CTGF expression in rat osteocytes. J.Dent.Res., 80,
461-465, 2001.
107) Oka, T., Yoshino, T.,Hayashi,K., Ohara,
N., Nakanishi, T., Yamaai, Y., Hiraki, A., Aoki-Sogawa, C.,Kondo,E., Teramoto,
N., Takahashi, K., Tsuchiyama, J. and Akagi, T.:Reducionofhematopoietic
cell-specific tyroshine phosphatase Shp-1 gene expressioninnatural killer cell
lymphoma and barious typesoflymphomas/leukemias:Combination analysis with cDNA
expression array andtissuemicyoarra., American Journal of Pathology, 159,
1495-1505, 2001.
108) Pan, L-H.,Yamauchi, M., Uzuki,M., Nakanishi, T., Takigawa, M., Inoue, H.
and Sawai,T.:Type ・ albeolar epithelialcells andinterstitial fibroblasts
express connective tissue growth factor inIPF. Eur.Respir. J. 17, 1220-1227,
2001
109) Suzuki, M., Kobayashi, H.,Fujie,M.,
Nishida, T., Takigawa, M., Kanayama, N. and Terao, T.:
Kunitz-typeproteaseinhibitor bikunin disrupts phorbol ester-induced
oligomerization ofCD44 variantisoforms containing epitope v9 and subsequently
suppressesexpression ofurokinase-type plasminogen activator in human
chondrosarcomacells. The Journalof Biological Chemistry, 277, 8022-8032, 2002
110) Sironen, R. K.,Karjalainen,H. M.,
Torronen, K., Elo, M. A., Kaarniranta, K., Takigawa, M.,Helminen, H.J.and
Lammi, M. J.: High pressure effects on cellular expressionprofile andmRNA
stability. A cDNA array analysis. Biorheology, 39, 111-117,2002.
111) Kondo, S., Kubota, S.,Shimo,T., Nishida,
T., Yoshimichi, G., Eguchi, T., Sugahara, T. and Takigawa,M.:Connective tissue
growth factor increased by hypoxia may initiateangiogenesisin collaboration
with matrix metalloproteinases. Carcinogenesis,23, 769-776,2002.
112) Sakata, M., Masuko-Hongo,K.,Tsuruha, J., Sekine, T., Nakamura, H., Takigawa,
M., Nishioka, K. and Kato,T.:YKL-39, a human cartilage-related protein,
induces arthritis in mice. Clin Exp Rheumatol., 20, 343-50, 2002
113) Tsuruha, J., Masuko-Hongo,K.,Kato, T.,
Sakata, M., Nakamura, H., Sekine, T., Takigawa, M. and Nishioka,K.:Autoimmunity
against YKL-39, a human cartilage-deribed protein in
patientswithosteoarthritis. J. Rheum., 29, 1459-66, 2002.
114) Nishida, T., Kubota,S.,Nakanishi, T.,
Kuboki, T., Yosimichi, G., Kondo, S. and Takigawa,M.:CTGF/Hcs24, a hypertrophic
chondrocyte specific gene product, stimulatestheproliferation and expression of
the cartilage phenotype but not hypertrophyorcalcification or articular
cartilage in culture. J Cell. Physiol., 192,55-63,2002.
115) Eguchi, T., Kubota, S.,Kondo,S., Kuboki,
T., Yatani, H. and Takigawa, M.: A novel cis-element thatenhancesconnective
tissue growth factor gene expression in chondorocyticcells. Biochem.Bophys.
Res. Commun., 395, 445-451, 2002.
116) Fang, J., Kubota, S.andPomerantz, R. J.: A
trans-dominant negative HIV-1 Rev with intact domainsofNLS/NOS and NES. AIDS
Res.& Hum. Retro., 18, 705-709, 2002.
117) Sironen, R. K.,Karjalainen,H. M., Elo,
M.A., Kaarniranta, K., Torronen, K., Takigawa, M.,Helminen, H. J.and Lammi, M.
J.: cDNA array reveals mechanosensitive genes inchondrocyticcells under
hydrostatic pressure. Biochim Biophys Acta 19, 45-54,2002.
118) Suzuki, M., Kobayashi,H.,Fujie., M,
Nishida, T., Takigawa, M., Kanayama, N. and Terao, T.:Kunitz-typeprotease
inhibitor bikunin disrupts phorbol ester-inducedoligomerization ofCD44 variant
isoforms containing epitope v9 and subsequentlysuppressesexpression of
urokinase-type plasminogen activator in humanchondrosarcomacells. J. Biol.
Chem., 277, 8022-8032, 2002.
119) Kobayashi, H., Suzuki,M.,Kanayama, N.,
Nishida, T., Takigawa, M. and Terao, T.: Suppression ofurokinasereceptor
expression by bikunin is associated with inhibition ofupstreamtargets of
extracellular signal-regulated kinase-dependent cascade.Eur. J.Biochem., 269,
16, 3945-3957, 2002.
120) Suzuki, M., Kobayashi,H.,Kanayama, N.,
Nishida, T., Takigawa, M. and Terao, T.: CD44 stimulation
byfragmentedhyaluronic acid induces upregulation and tyrosine phosphorylation
ofc-Metreceptor protein in human chondrosarcoma cells. Biochim. Biophys.Acta.,1591,37-44,
2002.
121) Yamamoto, Y., FurukawaK.,Ueyama, K.,
Nakanishi, T., Takigawa, M. and Harata, S.: Possible rolesofCTGF/Hcs24 in the
initiation and development of ossificationlongitudinalligament. Spine. 27,
1852-1857, 2002.
122) Nakata, E., Nakanishi,T.,Kawai, A.,
Asaumi, K., Yamaai, T., Asano, M., Nishida, T., Mitani, S.,Inoue,H. and
Takigawa, M.: Expression of connective tissue
growthfactor/hypertrophicchondorocyte-specific gene product 24(CTGF/Hcs24)
duringfracture healing.Bone, 31, 441-447, 2002.
123) Nawachi, K., Inoue,M.,Kubota, S., Nishida,
T., Yosimichi, G., Nakanishi, T., Kanyama, M., Kuboki,T.,Yatani, H., Yamaai, T.
and Takigawa, M.: Tyrosine kinase-type receptor ErbB4inchondrocytes:
interaction with connective tissue growth factor anddistributionin cartilage.
FEBS Lett., 528, 109-13, 2002.
124) Inoue, T., Okada,H.,Kobayashi, T.,
Watanabe, Y., Kikuta, T., Kanno, Y., Takigawa, M. andSuzuki,H.: TGF-beta1 and
HGF coordinately facilitate collagen turnoverinsubepithelial mesenchyme.
Biochem. Biophys. Res. Commun., 297, 255-260, 2002.
125) Kobayashi, H., Suzuki,M.,Kanayama, N.,
Nishida, T., Takigawa, M. and Terao, T.: CD44 stimulationbyfragmented
hyaluronic acid induces upregulation of urokinase-typeplasminogenactivator and
its receptor and subsequently facilitates invasion ofhumanchondrosarcoma cells.
Int. J. Cancer, 102, 379-389, 2002.
126) Yosimichi, G., Kubota,S.,Hattori, T., Nishida, T., Nawachi K, Nakanishi,
T., Kamada, M,Takano-Yamamoto,T and Takigawa, M.: CTGF/Hcs24 interacts
with the cytoskeletoalprotein actinin chondrocytes. Biochem.Bophys.
Res.Commun., 299, 755-761, 2002.
127) Shimizu, H.,Nishitani, Y.,Yamada, T., Nishida, T., Takigawa, M. and
Yoshiyama, M.:Development of dentinregeneration therapy: expression of
type I collagen andalkaline phosphataseinduced by CTGF in human cultured
dental pulp. J. HardTissue Biology, 11,62-67, 2002.
128) Karjalainen, H. M.,Sironen,R.K., Elo, M.
A., Kaarniranta, K., Takigawa, M., Helminen, H. J. andLammi, M.J.: Gene
expression profiles in chondrosarcoma cells subjected tocyclicstretching and
hydrostatic pressure. A cDNA array study. Biorheology,40,93-100, 2003.
129) Inoue, T., Okada, H.,Kobayashi,T.,
Watanabe, Y., Kanno, Y., Kopp, J. B., Nishida, T., Takigawa, M.,Ueno,
M.,Nakamura, T. and Suzuki, H.: Hepatocyte growth factor
counteractstransforminggrowth factor-beta1, through attenuation of connective
tissue growthfactorinduction, and prevents renal fibrogenesis in 5/6
nephrectomized mice.FASEBJ., 17, 268-270, 2003.
130) Mukudai, Y., Kubota, S.,andTakigawa, M.:
Conserved repressive regulation of connective tissue growthfactor/hypertrophic
chondrocyte-specific gene 24 (ctgf/hcs24) enabled bydifferentelements and
factors among vertebrate species. Biol. Chem., 384, 1-9,2003.
131) Brigstock DR,Goldschmeding R, Katsube KI, Lam SC, Lau LF, Lyons K,
Naus C, Perbal B,Riser B,Takigawa M, Yeger H.: Proposal for a unified CCN
nomenclature. MolPathol.56(2), 127-8, 2003.
132) Moritani, N. H., Kubota,S.,Kondo, S.,
Nishida, T., Kawaki, H., Sugahara, T. and Takigawa, M:Suppressiveeffect of
overexpressed connective tissue growth factor on tumorcell growth ina human
oral squamous cell carcinoma-derived cell line. CancerLett., 192(2),205-14,
2003.
133) Nishida, T., KubotaS.,Fukunaga, T., Kondo,
S., Yosimichi, G., Nakanishi, T., Takano-Yamamoto, T.andTakigawa, M.:
CTGF/Hcs24, hypertrophic chondrocyte-specific geneproduct,interacts with
perlecan in regulating the proliferation anddifferentiation ofchondrocytes. J.
Cell. Physiol., 196(2), 265-75, 2003.
134) Moritani, N. H., Kubota,S.,Eguchi, T.,
Fukunaga, T., Yamashiro, T., Takano-Yamamoto, T., Tahara,H.,Ohyama, K.,
Sugahara, T. and Takigawa, M.: Interaction of AP-1 and thectgfgene: a possible
driver of chondrocyte hypertrophy in growth cartilage. J.BoneMiner. Metab.,
21(4), 205-10, 2003.
135) 縄稚久美子：マクロファージコロニー刺激因子受容体遺伝子(m-csfr/c-fms)の軟骨細胞における発現. 岡山歯学会誌, 22, 83-89,
2003.
136) Kuboki, T., Kanyama,M.,Nakanishi, T.,
Akiyama, K., Nawachi, K., Yatani, H., Yamashita,K.,Takano-Yamamoto, T. and
Takigawa, M.: Cbfa1/Runx2 gene expression inthetemporomandibular and knee joint
articular chondrocytes in vivo. Archs. ofOralBiol., 48(7), 519-25, 2003.
137) Hattori, T., Kawaki,H.,Kubota, S., Yutani,
Y., de Crombrugghe, B., von der Mark, K., and Takigawa,M.:Downregulation of a
rheumatoid arthritis-related antigen (RA-A47) byra-a47antisense
oligonucleotides induces inflammatory factors in chondrocytes.J.Cell. Physiol.,
197, 94-102, 2003.
138) Fujisawa, T.,Kuboki, T., Kasai, T., Sonoyama, W., Kojima, S., Uehara,
J., Komori, C., Yatani,H.,Hattori, T. and Takigawa, M.: A repetitive steady
mouth openinginducedosteoarthritis-like lesion in rabbit temporomandibular
joint. J. Dent.Res.,82, 731-735, 2003.
139) Kanyama, M., Kuboki,T.,Akiyama, K.,
Nawachi, K., Miyauchi, F. M., Yatani, H., Kubota, S.,Nakanishi,T. and Takigawa,
M.: Connective Tissue Growth Factor Expressed in RatAlveolarBone Regeneration
Sites after Tooth Extraction. Arch. Oral Biol., 48,723-730,2003.
140) Kawaki, H., Kubota,S.,Minaro, M.,
Moritani, N. H., Hattori, T., Hanagata, H., Kubota, M.,Miyauchi,A., Nakanishi,
T. and Takigawa, M.: Novel enzyme-linked immunosorbentassaysystems for the
quantitative analyses of connective tissue growthfactor(CTGF/Hcs24/CCN2):
Detection of HTLV-1 tax-induced CTGF from a humancarcinomacell line. DNA Cell
Biol., 22, 641-648, 2003.
141) Fukunaga, T., Yamashiro,T.,Oya, S.,
Takeshita, N., Takigawa, M. and Takano-Yamamoto, T.: Connectivetissuegrowth
factor mRNA expression pattern in cartilages is associated withtheirtype I
collagen expression. Bone, 33(6), 911-8, 2003.
142) Kubota, S., Moritani, N.H.,Kawaki, H., Mimura, H., Minato, M. and
Takigawa, M.: Transcriptionalinductionof connective tissue growth factor/
hypertrophic chondrocyte specific24(CTGF/Hcs24) gene by dexamethasone in
human chondrocytic cells. Bone,33:694-702, 2003.
143) Yokoi, H., Mukoyama, M.,Nagae,T., Mori,
K., Suganami, T., Sawai, K., Yoshioka, T., Koshikawa, M.,Nishida, T.,Takigawa,
M., Sugawara, A. and Nakao, K.: Reduction in connectivetissue growthfactor by
antisense treatment ameliorates renaltubulointerstitial fibrosis. J.Am. Soc.
Nephrol., 15, 1430-40, 2004.
144) Minato, M., Kubota,S.,Kawaki, H., Nishida, T., Miyauchi, A., Hanagata,
H., Kubota, M., Nakanishi,T.,Yamamoto-Takano, T. and Takigawa, M.: Module-specificantibodiesagainst
human connective tissue growth factor: Utility for structuralandfunctional
analysis of the factor as related to chondrocytes. J. Biochem.135347-354.
2004.
145) Nishida, T., Kubota,S.,Kojima, S., Kuboki,
T., Nakao, K., Kushibiki, T., Tabata, Y. and Takigawa.M.: Regenerationof
defects in articular cartilage in rat knee joints by CCN2(connective
tissuegrowth factor). J. Bone Miner. Res., 19, 1308-1319, 2004.
146) Kadota, H., Nakanishi, T., Asaumi,K., Yamaai, T., Nakata,E., Mitani, S.,
Aoki, K., Aiga, A., Inoue, H andTakigawa, M.: Expression ofConnective Tissue
Growth Factor/HypertrophicChondrocyte-Specific Gene Product24 (CTGF/Hcs24/CCN2)
During DistractionOsteogenesis. J. Bone Miner. Metab.,22(4), 293-302, 2004.
147) Jung, y., Jong, J., Ryoo, H.,Kim, H., Kim, Y., Park, E., Si, H., Kim,S.,
Takigawa, M., Lee, B., Park, R,Kim, I., Choi, J.: Geneexpression profile
of human chondrocyte HCS 2/8 cell line by ESTsequencinganalysis. Gene,
330, 85-92, 2004.
148) Kaji, T., Yamamoto, C, Oh-I, M., Nishida, T. andTakigawa, M.: Differentialregulation
of biglycan and decorin synthesis byconnective tissue growth factorin cultured
vascular endothelial cells. Biochem BiophysResCommun., 322, 22-28, 2004
149) Hattori, T., von der Mark,K.,Kawaki, H.,
Yutani, Y., Kubota, S., Nakanishi, T., Eberspeacher, H.,deCrombrugghe, B. and
Takigawa, M.: Downregulation ofrheumatoidarthritis-related antigen RA-A47 (=HSP47/Colligin-2)
in chondrocyticcell linesinduces apoptosis and cell-surface expression of
RA-A47 inassociation withCD9. J. Cell. Physiol., 197(1), 94-102, 2004.
150) Gebhard, S., Pöschl,E., Riemer, S., Bauer, E., Hattori, T., Eberspaecher,
H., Zhang, Z., Lefebvre,V., de Crombrugghe, B. and von der Mark, K. A.:
Highly Conserved Enhancer inMammalian Type X Collagen Genes Drives High
Levels of Tissue-SpecificExpression in Hypertrophic Cartilage in vitro
and in vivo. Matrix Biol., 23(5),309-322, 2004.
151) Fushimi, K.,Nakashima, S., Takigawa, M. and Shimizu, K.: Implication
of prostaglandin E2 inTNF-a-induced release of m-calpain from HCS-2/8 chondrocytes:
Inhibition ofm-calpain release by NSAIDs Osteoarthritis and Cartilage,
12(11), 895-903,2004.
152) Omoto, S.,Nishida, K., Shibahara, M., Nishida, T., Doi, T., Asahara, H.,
Nakanishi, T.,Inoue, H. and Takigawa, M.: Expression and localization of
Connective tissuegrowth factor (CTGF/Hcs24/CCN2) by chondrocytes in osteoarthritic
cartilage.Osteoarthirtis and Cartilage, 12(10), 771-778, 2004.
153) Kubota, S.,Kawata, K., Yanagita, T., Doi, H., Kitoh, T. and Takigawa, M.:
Abundant retention releaseof connective tissue growth factor (CTGF/CCN2)
by platelets. J. Biochem. 136,279-82, 2004.
154) Saas J., LindauerK., Bau B., Takigawa M.,
and Aigner T.: Molecular phenotyping of HCS-2/8 cellsas an in vitro model of
human chondrocytes. Osteoarthritis Cartilage 12(11),924-34, 2004.
155) Fang, J., Kubota,S., Yang, B., Zhou, N., Zhang, H., Godbout, R. and Pomerantz,
R.J.: A DEAD boxprotein facilitates HIV-1 Replication as a cellular co-factor
of Rev. Virology.330, 471-480, 2004.
156) Hattori, T., von der Mark, K., Kawaki, H., Yutani, Y., Kubota, S.,
Nakanishi, T., Eberspeacher, H.,de Crombrugghe, B. and Takigawa, M. Downregulation
of rheumatoidarthritis-related antigen RA-A47 (=HSP47/Colligin-2) in chondrocytic
cell linesinduces apoptosis and cell-surface expression of RA-A47 in association
withCD9. J. Cell. Physiol., 202, 191-204, 2005.
157) Mukudai, Y.,Kubota, S., Eguchi, T., Kondo, S., Nakao, K. and Takigawa,
M.: Regulation ofchicken ccn2 gene by interaction between RNA cis-element
and trans-factorduring differentiation of chondrocytes. J. Biol. Chem.,
280, 3166-3177, 2005.
158) Okada, H., Kikuta,T., Kobayashi, T.,
Inoue, T., Kanno, Y., Takigawa, M., Sugaya, T., Kopp, J.B.and Suzuki, H:
Connective tissue growth factor expressed in tubular epitheliumplays a pivotal
role in renal fibrogenesis. J. Am. Soc. Nephrol. 16(1), 133-143,2005.
159) Chujo, S.,Shirasaki, F., Kawara, S.,
Inagaki, Y., Kinbara, T., Inaoki, M., Takigawa, M.and Takehara, K.: Connective
tissue growth factor causes persistent proa2(I)collagen gene expression induced
by transforming growth factor-b in a mousefibrosis model. J. Cell. Physiol.
203(2), 447-456, 2005.
160) Gebauer, M., Saas,J., Haag, J., Dietzb, U., Takigawa, M., Aigner, T.: Repression
ofanti-proliferative factor Tob1 in osteoarthritic cartilage. Arthritis
Research Therapy, 7, R274-R284,2005.
161) Chrysis, D., Zaman, F., Chagin, AS., Takigawa, M., Sävendahl, L.:
Dexamethasone induces apoptosis in proliferative chondrocytes throughactivation
of caspases and suppression of the Akt-(PI3K) signalling pathway. Endocrinology,
46(3), 1391-1397, 2005.
162) Nakao, K., Kubota,S., Doi, H., Eguchi, T., Oka, M., Fujisawa, T.,
Nishida, T. and Takigawa, M.:Collaborative action of M-CSF and CTGF/CCN2
in articular chondrocytes:possibleregenerative roles in articular cartilage
metabolism. Bone, 36, 884-892, 2005.
163) Yamaai, T.,Nakanishi, T., Asano, M.,
Nawachi, K., Yosimichi, G., Ohyama, K., Komoari, T.,Sugimoto, T. and Takigawa,
M.: Gene expression of connective tissue growthfactor (CTGF/CCN2) in calcifying
tissues of normal and cbfa1-null mutant inlate stage of mouse embryonic
development. J. Bone Miner. Metab., 23, 280-288,2005.
164) Moritani, NH.,Kubota, S., Sugahara, S. and Takigawa, M.: Comparable response
of ccn1 with ccn2 genes uponarthritis: An in vitro evaluation with a human
chondrocytic cell linestimulated by a set of cytokines. Cell Communication
and Signaling, 3(1), 6,2005.
165) Tsuchiya, T.,Osanai, T., Ogose, A.,
Tamura, G., Chano, T., Kaneko, Y., Ishikawa, A., Orui,H., Wada, T., Ikeda, T.,
Namba, M., Takigawa, M., Kawashima, H., Hotta, T.,Tsuchiya, A., Ogino, T. and
Motoyama, T.: Methylation status of EXT1 and EXT2promoters and two mutations of
EXT2 in chondrosarcoma. Cancer Genet Cytogenet.158(2), 148-155, 2005.
166) Kubota, S.,Mukudai, Y., Moritani, N.H., Nakao, K, Kawata, K. and K
Takigawa, M.: Transcriptionalrepression by the cis-acting element of structure-anchored
repression (CAESAR)of human ctgf/ccn2 mRNA. FEBS Lett, 579, 3751-3758,
2005.
167) Kobayashi, T.,Inoue, T., Okada, H.,
Kikuta, T., Kanno, Y., Nishida, T., Takigawa, M. Sugaya,T. and Suzuki, H.:
Connective tissue growth factor mediates the profibroticeffects of transforming
growth factor-beta produced by tubular epithelial cellsin response to high
glucose. Clin Exp Nephrol. 9(2), 114-121, 2005.
168) Saas, J., Gebauer,M., Jacobi, C., Haag, J., Takigawa, M., Aigner, T.: The
human chondrosarcomaHCS-2/8 cell line is responsive to BMP-7, but not to
IL-1beta. Front. Biosci., 10, 2027-2035, 2005.
169) Asano, M., Kubota,S., Nakanishi, T., Nishida, T., Yamaai, T., Yosimichi,
G., Ohyama, K.,Sugimoto, T., Murayama, Y. and Takigawa, M.: Effect of connective
tissue growthfactor (CCN2/CTGF) on proliferation and differentiation of
mouse periodontalligament-derived cells. Cell Commun Signal. 2005 Oct 5;3(1):11（Epub
Journal）
170) Hong Mei Piao, Kohei yamauchi, Li-Hua Pan, Toshihide Nakadate, Harumasa
Ito, TakashiMouri, Hitoshi Kobayashi, Takashi Sawai, Tohru Nakanishi, Masaharu
Takigawa and HiroshiInoue: Increased Levels of CTGF mRNA Expression in
a Murine Model of AllergicAirway Inflammation. Allergology International,
54, 107-115, 2005.
171) Maeda, T., Jikko, A., Abe, M., Yokohama-Tamaki, T., Akiyama, H.,Furukawa,
S., Takigawa, M. and Wakisaka, S.: Cartducin, a Paralog ofacrp30/adiponectin,
is induced during chondrogenicdifferentiation and promotes proliferation
of chondrogenicprecursors and chondrocytes. J. Cell. Physiol., 206(2),
537-544, 2005.
172) Kondo, S., Kubota,S., Mukudai, Y., Moritani, N., Nishida, T., Matsushita,H.,
Matsumoto, S., Sugahara, T. and Takigawa, M.: Hypoxic regulationof stability
of connective tissue growth factor/CCN2 mRNA by 3’-untranslatedregion interacting
with a cellular protein in human chondrosarcoma cells.Oncogene, 25(7):
1099-1110, 2006.
173) Kondo, S., Tanaka,N., Kubota, S., Mukudai,
Y., Yosimichi, G., Sugahara T., and Takigawa, M.:Novel angiogenic inhibitor
DN-9693 that inhibits post-transcriptional inductionof connective tissue growth
factor (CTGF/CCN2) by vascular endothelial growthfactor (VEGF) in human
endothelial cells. Molecular Cancer Therapeutics 5,129-137, 2006.
174) Yosimichi, G.,Kubota, S., Nishida, T.,
Kondo, S., Yanagita, T., Nakao, K., Takano-Yamamoto,T. and Takigawa, M.: Roles
of PKC, PI3K and JNK in multiple transduction ofCCN2/CTGF signals in
chondrocytes. Bone, 38, 853-863, 2006.
175) Hoshijima M., Hattori, MT., Inoue,M.,Araki, D, Hanagata, H.Miyauchi, A.,
and Takigawa, M. CT domain of CCN2/CTGF directly interacts withfibronectin
and enhances cell adhesion of chondrocytes integrin a5b1. FEBS Lett. 580(5),1376-1382,
2006.
176) Okada, H., Kikuta,T., Inoue, T., Kanno,
Y., Ban, S., Sugaya, T., Takigawa, M., and Suzuki, H.:Dexamethasone induced connective
tissue growth factor expression in renaltugbular epithelial cells in a mouse
strain-specific manner. Am. J. Pathol.168(2), 737-743, 2006.
177) Chagin, A. S.,Chrysis, D., Takigawa, M.,
Ritzen, E. M., and SavendahI, L. Locally producedestrogen promotes fetal rat
metatarsal bone growth; an effect mediated throughincreased chondrocyte
proliferation and decreased apoptosis. J. Endocrinol.,188(2), 193-203, 2006.
178) Kubota, S., andTakigawa, M. CCN family
genes in the development and differentiation ofcartilage tissues. Clin.
Calcium, 16(3), 486-492, 2006, Japanese.
179) Kawata, K.,Eguchi, T., Kubota, S., Kawaki, H., Oka, M., Minagi, S.,
and Takigawa, M. :Possible role of LRP1, a CCN2 receptor, in chondrocytes.
Biochem Biophys Res Commun., 345(2):552-559,2006.
180) Hattori, T.,Eberspaecher, H., Lu, J., Zhang, R., Nishida, T., Kahyo, T., Yasuda, H., deCrombrugghe, B. : Interactions between PIAS proteins and SOX9 results in anincrease in the cellular concentrations of SOX9. J. Biol. Chem.,281(20):14417-14428, 2006.
181) Kubota, S., Kawaki, H.,Kondo, S.,
Yosimichi, G., Minato, M., Nishida, T., Hanagata, H., Miyauchi, A.,Takigawa, M.
Multiple activation of mitogen-activated protein kinases bypurified independent
CCN2 modules in vascular endothelial cells andchondrocytes in cultue. Biochimie,
in press, 2006.
182) Shimo, T., Kubota, S.,Eguchi, T., Ibaragi,
S., Isowa, S., Yoshioka, N., Takigawa, M., Sasaki, A.Pathogenic role of
connective tissue growth factor (CCN2/CTGF) in osteolyticmetastasis of breast
cancer. J. Bone Miner. Res., 21:1045-1059, 2006.
183) Schmidl M., Adam N., Surmann-Schmitt C., Hattori T., Stock M., Dietz
U., de CrombruggheB., Pöschl E., and von der Mark K. Twisted gastrulation
modulates bonemorphogenetic protein-induced collagen II and X expression
in chondrocytes invitro and in vivo. J. Biol. Chem. 281(42): 31790-31800,
2006.
184) Kondo,S., Kubota, S., Sugahara, T. and Takigawa, M. Expression and regulation
of ananti-sense RNA transcript from human connective tissue growth factor
gene inhuman cells. Asian J. Oral Mallilofa.Surg., 18: 172-179, 2006.
185) AigaA., Asaumi K., Lee Y. J., Kadota H., Mitani S., Ozaki T., Takigawa
M. Expression of neurotrophins and theirreceptors tropomyosin-related
kinases (Trk) under tension-stress duringdistraction osteogenesis.. Acta
Med Okayama., 60(5):267-277, 2006.
186) Mori M., Nakajima M., Mikami Y., Seki S., Takigawa M., Kubo T., Ikegawa
S.Transcriptional regulation of the cartilage intermediate layer protein
(CILP)gene. Biochem. Biophys. Res. Commun.341, 121-127, 2006.
187) GebhardS., Hattori T., Bauer E., Bosl M.R., Schlund B., Poschl E., Adam
N., de CrombruggheB., von der Mark K. BAC constructs in transgenic reporter
mouse linescontrol efficient and specific LacZ expression in hypertrophic
chondrocytesunder the complete Col10a1 promoter. Histochem Cell Biol.,
127(2): 183-194,2007.
188) ZamanF, Menendez-Benito V, Eriksson E, Chagin AS, Takigawa M, Fadeel
B, Dantuma NP,Chrysis D and Savendahl L Proteasome inhibition up-regulates
p53 andapoptosis-inducing factor in chondrocytes causing severe growth
retardation inmice. Cancer Res. 67(20): 10078-10086, 2007.
189) HiranoY, Ishiguro N, Sokabe M, Takigawa M
and Naruse K Effects of tensile andcompressive strains on response of a
chondrocytic cell line embedded in type Icollagen gel. J. Biotechnol. 133(2):
245-252, 2007.
190) FushimiK., Nakashima S.,You F., Takigawa
M., Shimizu K. Prostaglandin E(2)downregulates TNF-alpha-induced production of
matrix metalloproteinase-1 inHCS-2/8 chondrocytes by inhibiting Raf-1/MEK/ERK
cascade through EP4 prostanoidreceptor activation. J. Cell Biochem. 100(3):
783-793, 2007.
191) Hattori T. Takigawa M. Autoantigen and molecularchaperone in rheumatoid
arthritis--their roles in metabolism ofchondrocytes] Clin Calcium.
16(9):133-136, 2006, Japanese.
192) ChaginA. S., Karimian E., Zaman F.,
Takigawa M., Chrysis D. and Savendahl L. Tamoxifeninduces permanent growth
arrest through selective induction of apoptosis ingrowth plate chondrocytes in
cultured rat metatarsal bones. Bone, 40(5),1415-1424, 2007.
193) YanagitaT., Kubota S., Kawaki H., Kawata
K., Kondo S., Takano-Yamamoto T., Tanaka S.,Takigawa M. Expression and
physiological role of CCN4/Wnt-induced secretedprotein 1 mRNA splicing variants
in chondrocytes. FEBS J. 274(7): 1655-1665,2007.
194) OnoM., Kubota S., Fujisawa T., Sonoyama
W., Kawaki H., Akiyama K., Ohima M.,Nishida T., Yoshida Y., Suzuki K., Takigawa
M., Kuboki T. Promotion ofattachment of human bone marrow stromal cells by
CCN2. Biochem Biophys ResCommun. 357(1): 20-25, 2007.
195) KoitabashiN., Arai M., Kogure S., Niwano
K., Watanabe A., Aoki Y., Maeno T., Nishida T.,Kubota S., Takigawa M.,
Kurabayashi M. Increased connective tissue growthfactor relative to brain
natriuretic Peptide as a determinant of myocardialfibrosis. Hypertension.
49(5): 1120-1127, 2007.
196) EguchiT., Kubota S., Kawata K., Mukudai
Y., Ohgawara T., Miyazono K., Nakao K., KondoS., Takigawa M. Different
transcriptional strategies for ccn2/ctgf geneinduction between human
chondrocytic and breast cancer cell lines. Biochimie.89(3): 278-288, 2007.
197) NishidaT., Kawaki H., Baxter R.M., DeYoung
A., Takigawa M., Lyons K.M. CCN2(Connective tissue growth factor) is essential
for extracellular matrixproduction and integrin signaling in chondrocytes. J
Cell Commun Signal, 1,45-58, 2007.
198) MiyamatoY., Mabushi A., Shi D., Kubo T.,
Takatori Y., Saito S., Fujioka M., Sudo A.,Uchida A., Yamamoto S., Ozaki K.,
Takigawa M., Tanaka T., Nakamura Y., JiangQ., Ikegawa S. A functional
polymorphism in the 5’ UTR of GDF5 is associatedwith susceptibility to
osteoarthritis. Nat. Genet. 39, 529-533, 2007.
199) OkaM., Kubota S., Kondo S., Eguchi T.,
Kuroda C., Kawata K., Minagi S., TakigawaM. Gene expression and distribution of
connective tissue growth factor(CCN2/CTGF) during secondary ossification center
formation. J. Histochem.Cytochem., 55: 1245-1255, 2007.
200) OnoM., Kubota S., Fujisawa T., Sonoyama
W., Kawaki H., Akiyama K., Oshima M.,Nishida T., Yoshida Y., Suzuki K.,
Takigawa M., Kuboki T. Promotion ofhydroxyapatite-associated, stem cell-based
bone regeneration by CCN2. CellTransplantation, 17(1-2), 231-240, 2008.
201) MatsumotoY., Motoki T., Kubota S., Takigawa M., Tsubouchi H., Gohda E.
Inhibition of tumor-stromal interactionthrough HGF-Met signaling by valproic
acid. Biochem. Biophys. Res. Commun., 366(1):110-116, 2008.
202) DoiH., Nishida K., Yorimitsu M., Komiyama T., Kadota Y., Tetsunaga T.,
Yoshida A.,Kubota S., Takigawa M., Ozaki T. Interleukin-4 downregulates
the cyclic tensilestress-induced matrix metalloproteinases-13 and cathepsin
B expression by ratnormal chondrocytes. Acta MedicaOkayama, 62(2),
119-126, 2008.
203) EguchiT, Kubota S, Kawata K, Mukudai Y, Uehara J, Ohgawara T, Ibaragi S,
Sasaki A,Kuboki T, Takigawa M. Novel transcription factor-like function
of human MMP3regulating CTGF/CCN2 gene. Mol. Cell.Biol., 28: 2391-2413,
2008.
204) KoitabashiN., Arai M., Niwano K., Watanabe A., Endoh M., Suguta M., Yokoyama
T., Tada H.,Toyama T., Adachi H., Naito S., Oshima S., Nishida T., Kubota
S., Takigawa M.,Kurabayashi M. Plasma connective tissuegrowth factor
is a novel potential biomarker of cardiac dysfunction in patientswith chronic
heart failure. Eur. J. Heart Failure, 10(4): 373-379, 2008.
205) FujisawaT., Hattori T., Ono M., Uehara J.,
Kubota S., Kuboki T., Takigawa M. CCN family2 / Connective tissue growth factor
(CCN2/CTGF) stimulates proliferation anddifferentiation of auricular
chondrocytes. Osteoarthritis and Cartilage, 16(7),787-795, 2008.
206) KawakiH., Kubota S., Suzuki A., Yamada T.,
Matsumura T., Mandai, T., Yao, M., MaedaT., Lyons K.M., Takigawa M. Functional
requirement of CCN2 for intramembranousbone formation in embryonic mice.
Biochem. Biophys. Res. Commun., 366(2):450-456, 2008.
207) KawakiH., Kubota S., Suzuki A., Lazar, N.,
Yamada, T., Matsumura, T., Ohgawara, T.,Maeda, T., Perbal, B. Lyons, K. M.,
Takigawa M. Cooperative regulation ofchondrocyte differentiation by CCN2 and
CCN3 shown by a comprehensive analysisof the CCN family proteins in cartilage.
J. Bone Miner. Res. 23(11): 1751-1764,2008.
208) NishidaT., Maeda A., Kubota S., Takigawa
M. Role of mechanical-stress inducibleprotein Hcs24/CTGF/CCN2 in cartilage
growth and regeneration: -Mechanicalstress induces expression of
Hcs24/CTGF/CCN2 in human chondrocytic cell line,HCS-2/8, rabbit hyaline costal
chondrocytes and meniscus cells- Biorheology,45(3-4), 289-299, 2008.
209) SunG., Haginoya K., Wu Y., Chiba Y.,
Nakanishi T., Onuma A., Sato Y., Takigawa M.,Iinuma K., Tsuchiya S. Connective
tissue growth factor is overexpressed inmuscles of human muscular dystrophy. J
Neurol Sci. 267(1-2), 48-56, 2008.
210) KikuchiT., Kubota S., Asaumi K., Kawaki
H., Nishida T., Kawata K., Mitani S., TabataY, Ozaki T., Takigawa M. Promotion
of bone regeneration by CCN2 incorporatedinto gelatin hydrogel. Tissue
Engineering Part A 14(6), 1089-1098, 2008.
211) Hattori,T., Coustry, F., Stephens, S.,
Eberspaecher, H., Takigawa, M., Yasuda, H., deCrombrugghe, B. Transcriptional
regulation of chondrogenesis by coactivatorTip60 via chromatin association with
Sox9 and Sox5. Nucleic Acids Res. 36(9):3011-3024, 2008.
212) Motoki,T., Sugiura, Y., Matsumoto, Y.,
Tsuji, T., Kubota, S., Takigawa, M., Gohda, E.Induction of hepatocyte growth
factor expression by maleic acid in humanfibroblasts through MAPK activation.
J. Cell Biochem. 104(4): 1465-1476, 2008.
213) Kuroda,C., Kubota, S., Kawata, K., Aoyama,
E., Sumiyoshi, K., Inoue, M., Minagi, S.,Takigawa, M. Distribution, gene expression,
and functional role of EphA4 duringossification. Biochem. Biophys. Res. Commun.
374(1): 22-27, 2008.
214) Mukudai,Y., Kubota, S., Kawaki, H., Kondo,
S. Eguchi, T., Sumiyoshi, K., Ohgawara, T.,Shimo, T., Takigawa, M.
Post-transcriptional regulation of chicken ccn2 gene expression
bynucleophosmin/B23 during chondrocyte differentiation. Mol. Cell Biol.
28(19):6134-6147, 2008.
215) Shimo,T. Kubota, S., Goda, T., Yoshihama,
Y., Kurio, N., Nishida, T., Ng, P. S.,Endo, K., Takigawa, M. Sasaki, A.
Clinical significance and pathogenic functionof connective tissue growth factor
(CTGF/CCN2) in osteolytic mandibularsquamous cell carcinoma. Anticancer Res. 28
(4C): 2343-2348, 2008.
216) GebhardS., Hattori T., Bauer E., Schlund B., Bösl MR., deCrombrugghe
B., von der Mark K. Specific expression of Cre recombinase inhypertrophic
cartilage under the control of a BAC- Col10a1 promoter. MatrixBiol. 27(8):
693-699, 2008.
217) Takeuchi,H., Kubota, S., Murakashi, E., Fukada, T., Hashimoto, S., Takigawa,
M. andNumabe, Y.: Effect of TGF-β1 on CCN2/CTGF in normal human gingival
fibroblastsand periodontal ligament cells. J. periodontal. Res., 44: 161-169,
2009.
218) Nishida,T. Kondo, S., Maeda, A., Kubota,
S., Lyons, K. M., Takigawa, M. CCN family 2/ connective tissue growth factor
(CCN2/CTGF) regulatesthe expression of Vegf through Hif-1a expression in a
chondrocytic cell line,HCS-2/8, under hypoxic condition. Bone 44 (1): 24-31,
2009.
219) Maeda,A., Nishida, T., Aoyama, E., Kubota,
S., Lyons, K. M., Kuboki, T., Takigawa, M.CCN family 2/connective tissue growth
factor modulates BMP signaling as asignal conductor, which action regulayes the
proliferation and differentiationof chondrocytes. J. Biochem. 145(2): 207-216,
2009.
220) Ohgawara,T., Kubota, S., Kawaki, H.,
Kondo, S., Eguchi, T., Kurio, N., Aoyama, E.,Sasaki, A. Takigawa, M. Regulation
of chondrocytic phenotype by micro RNA 18a:Involvement of Ccn2/Ctgf as a major
target gene. FEBS Lett. 583: 1006-1010,2009.
221) AoyamaE, Hattori T, Hoshijima M, Araki D, Nishida T, Kubota S, Takigawa
M.:N-terminal domains of CCN protein 2/connective tissue growth factor
bind toaggrecan. Biochem. J., 420:413-420, 2009.
222) Sakai Y, Balam TA, Kuroda S, Tamamura N, Fukunaga T, Takigawa M, Takano-Yamamoto T.:CTGF and apoptosis in
mouse osteocytes induced by tooth movement. J Dent Res., 88(4):345-50, 2009.
223) Hikichi Y, Yoshimura K, Takigawa M. All-trans retinoic acid-induced ADAM28
degrades proteoglycans in human chondrocytes. Biochem Biophys Res Commun.
386(2): 294-299, 2009.
224) Hattori, T., Mueller, C., Gebhard, S., Bauer, E., Pausch, F., Schlund, B., Boesl, M., Hess, A.,
Surmann-Schmitt, C., von der Mark, H., de Crombrugghe, B., von der Mark. K. Sox9 is a major negative regulator
of cartilage vascularisation, bone marrow formation, and endochondral ossification. Development, 137, 901-911, 2010.
225) Kawata K, Kubota S, Eguchi T, Moritani NM, Shimo T, Kondo, S, Nishida T, Minagi S, Takigawa M.
Role of the low-density lipoprotein-1 in regulation of chondrocyte differentiation.
J. Cell. Physiol., 222:138-48, 2010
226) Sumiyoshi K, Kubota, S, Furuta, RA, Tasui K, Aoyama, E, Kawaki H, Kawata, K, Ohgawara T, Yamashiro T,
Takigawa M. Thrombopoietic-mesenchymal interaction that may facilitate both endochondral ossification
and pletelet maturation via CCN2. J. Cell Commun. Signal., 4, 5-14, 2010.
227) Lindahl E, Nyman U, Zaman F, Palmberg C, Cascante A, Shafqat J, Takigawa M, Savendahl L, Jornvall H,
Joseph B.: Proinsulin C-peptide regulates ribosomal RNA expression. J. Biol. Chem., 285(5): 3462-3469, 2010.
228) Takeuchi H, Kubota S, Murakashi E, Zhou Y, Endo K, Ng PS, Takigawa M, Numabe Y.: Nicotine-induced CCN2:
from smoking to periodontal fibrosis. J. Dent. Res., 89(1): 34-9, 2010.
229) 原哲也、兒玉（島津）千恵、河田かずみ、白嶋章、皆木省吾. 学生のリアルタイム評価を応用した授業内容改善FDシステムの開発. 日本歯科医学教育学会雑誌,
26, 77-81, 2010.
230) Kawaki,
H., Kubota, S., Aoyama, E., Fujita, N., Hanagata, H., Miyauchi, A., Nakai, K.,
Takigawa, M. Design and utility of CCN2 anchor peptide aptamers. Biochimie, 92,
1010-1015, 2010.
231) Sumiyoshi K, Kubota S, Ohgawara T, Kawata K, Nishida T, Shimo T, Yamashiro
T, Takigawa M. Identification of miR-1 as a micro RNA that supports late
stage differentiation of growth cartilage cells. Biochem. Biophys. Res.
Commun., 402, 286-290, 2010.
232)
Mukudai, Y., Kubota, S., Eguchi, T., Sumiyoshi, K., Janune, D., Kondo, S.,
Shintani, S. and Takigawa, M.: A coading RNA segment that enhances the
ribosomal revruitment of chicken Ccn1 mRNA.J
Cell Biochem. 111, 1607-1618, 2010.
233) Coustry, F., Oh, C., Hattori, T., Maity, S., de Crombrugghe, B. and Yasuda, H.: The dimerization domain of Sox9 is required for transcription activation of a chondrocyte-specific chromatin DNA template. Nucleic Acids Res. 38, 6018-6028, 2010.
