International Center for Structural Biology

Research Institute for Interdisciplinary Science, Okayama University

  • Japanese

ICSB Workshops
2nd ICSB Training Weekend

The International Center for Structural Biology (ICSB) is pleased to announce the 2nd ICSB Training Weekend. This introductory workshop is designed for beginners in single-particle analysis using cryo-electron microscopy in English.
Participants will learn the basic workflow through lectures and hands-on practice using tutorial datasets. The image-processing software CryoSPARC will be used during the training.
Although the hands-on training will be conducted in person, online viewing is also available.

Registration Form
https://docs.google.com/forms/d/e/1FAIpQLSfhZ7tQRY1f-3bvS1922yKUEj-0Dnx0-Z1iO5pX0WC4FXl_0Q/viewform?usp=dialog

Dates
Day 1: Thursday, September 3, 2026 — 13:00–17:45
Day 2: Friday, September 4, 2026 — 9:30–12:00
 (Information exchange session / individual consultation and Facility tour in the afternoon for interested participants)

Venue
Okayama University Tsushima Campus
Co-Creation Innovation Lab Building (KIBINOVE), 5th Floor — Connect Studio
(Information exchange session / individual consultation: 1st Floor Reception Area)
https://www.orsd.okayama-u.ac.jp/kigyo/kibinove/

Target Participants
Beginners with limited experience in protein structure analysis using cryo-electron microscopy.
Students, faculty members, and researchers from industry are all welcome.

Capacity
In-person: 15 participants
Online: 300 participants

Preparation for In-person Participants:
Please bring your own laptop.
No special setup is required, as the analysis environment will be accessed via a web browser.
We expect participants to connect to the university network using eduroam. If you cannot use eduroam, please inform us in advance.
Installing ChimeraX is recommended but not mandatory.
https://www.cgl.ucsf.edu/chimerax/

Language
English

Fee
Free
(There is no financial support for travel or accommodation expenses to the university.)

Accommodation Information
If you wish to stay at the Tsushima Lodgings
https://www.okayama-u.ac.jp/tp/profile/tsushima_c00.html
https://www.okayama-u.ac.jp/up_load_files/freetext/soumu-tsushima_c01/file/tsushima_c_knowledge_en2604.pdf
please indicate this on the registration form.
We will make a reservation on your behalf for a one-night stay on September 3 (please pay the accommodation fee yourself).
*Please be aware that the number of available rooms is limited. As rooms are not pre-allocated, we may not be able to secure a room for you even if you request one.

Instructor
TSAI, Pi-Cheng(ICSB, Okayama Univ.)
Nobutaka Numoto(ICSB, Okayama Univ.)

Program(Tentative)
September 3
13:00 - 13:30 Lecture: Overview of single particle analysis method
13:30 - 13:45 Login to the workstation
13:45 - 14:15 Motion correction, CTF estimation
14:15 - 14:45 Particle picking, extraction, 2D classification
14:45 - 15:15 Particle picking based on template matching、extraction, 2D classification
15:15 - 15:45 Break
15:45 - 16:15 Lecture: Overview of CTF and 3D reconstruction
16:15 - 16:45 Ab initio 3D reconstruction
16:45 - 17:15 Heterogeneous refinement、particle re-extraction
17:15 - 17:45 Homogeneous refinement

September 4
9:30 - 10:00 Model superposition using ChimeraX
10:00 - 10:30 Break
10:30 - 11:30 CTF refinement
11:30 - 12:00 Wrap-up

Afternoon Information exchange session / individual consultation and Facility tour for interested participants

Organized by
International Center for Structural Biology,
Research Institute for Interdisciplinary Science, Okayama University

Associated Programs
Program for Forming Japan's Peak Research Universities (J-PEAKS), Okayama University

Contact information
International Center for Structural Biology
staff.csb@okayama-u.ac.jp