大学院ヘルスシステム統合科学研究科　オルガネラシステム工学研究室

画像・プロトコル

Protocols

• Genome editing and activation by Crispr-Cas9 system
• Lentivirus production
• Retrovirus production
• Moviwol mounting solution preparation
• Tranasfection using PEI max
• Establishing stable line without virus
• 3D modの簡易日本語マニュアル（杓野さんの修論の一部）
• Organelle panel by immunofluorescence
• Reporter assay (OECD 442D related)
• cDNA prep for qPCR
• Melanin quantitation
• Auto-induction for protein prep

Images

• BFA treated Golgi apparatus
• H₂O₂ treated Golgi apparatus
• Nocodazole^TM treated Golgi apparatus
• Ilimaquinone treated Golgi apparatus
• Cytochalasin B treated Golgi apparatus
• Staurosporine treated Golgi apparatus
• MG132 treated Golgi apparatus

• Golgi and ER
• Golgi
• Golgi and ER
• Golgi and lysosomes
• Golgi and mitochondria

Stable lines

• SEAP expressing HeLa cell line for secretion assay
• GFP-Sec31A (ERES) expressing HeLa cell line
• ManII (Golgi)-GFP expressing HeLa cell line
• ManII (Golgi)-LgBiT expressing COS7 cell line
• ss-LgBiT-KDEL (ER) expressing COS7 cell line
• XRE-Nluc expressing HaCaT cell line
• ARE-Nluc expressing HaCaT cell line