哺乳動物の受胎性向上
哺乳動物において、黄体は妊娠を成立し維持していくのに必須の卵巣に形成される一過性の内分泌器官です。排卵後に必ず形成される黄体は、妊娠が成立し
なかった際次の排卵周期を回帰するために退行（消失）しなければなりません。黄体退行は、排卵周期の原点ともいえる重要な生殖現象であるにもかかわらず、そのメカニズムには不明な点が数多く残されています。私たちは、黄体退行機構に関与する黄体ならびに子宮の機能を生殖内分泌学的探索により解明し、黄体退行機構の全貌を明らかにすることを目的として研究しています。黄体退行機構を明らかにし、それらを制御する方法を開発することで、「排卵周期の人為的制御による家畜の効率的な生産法」、「不妊などの婦人病、生殖器がんの診断・治療法」を確立することができます。
卵管は卵巣と子宮の間に位置する細管であり、受精の場であるとともに、受精後数日の受精卵 (初期胚) の発育・成長を促し、さらには着床の場である子宮へ初期胚を輸送する役割を担う、妊娠の成立に不可欠な器官です。私たちはこれらの卵管内で起こる現象のメカニズムをより深く明らかにすることで、”哺乳動物の受胎性向上”のみならず、ヒト不妊症の
30% を占める”卵管障害の原因究明”の足掛かりとなることを目指し研究しています。
1) 黄体内局所調節因子による黄体退行機構の解明
黄体は、プロジェステロンを始め多くの生理活性物質を合成分泌する一過性の内分泌器官です。この黄体機能が、黄体内外のどのような因子によって制御されているかについて詳細に研究しています。
2) 子宮内膜のプロスタングランディン合成調節機構の解明
黄体が機能を失った後、黄体細胞のプログラムされた死 (アポトーシス) が誘導されることにより黄体組織が消失します。このアポトーシスを促進する因子と抑制する因子を明らかにし、アポトーシス調節機構の解明を目指しています。
3) サイトカインが黄体機能および子宮内膜機能に及ぼす影響
黄体退行が起こる直前、大量の免疫細胞が黄体と子宮に流入してきます。免疫細胞が分泌するサイトカインが、黄体および子宮内膜の機能にどのような影響を及ぼすかについて細胞と生体の両面から研究しています。
4) 子宮内膜のプロスタングランディン分泌調節因子の検索
子宮内膜におけるプロスタグランディン (PG) 類の分泌は、様々な生理活性物質により調節されると考えられています。どのような物質がどのようなシグナルを介して子宮内膜の PG 分泌調節に関わっているかを検索しています。
5) 卵管蠕動運動制御メカニズムの解明
卵管における蠕動運動調節因子の分泌は、性ホルモンを代表とする様々な生理活性物質よって制御されています。私たちはどのような物質が蠕動運動調節因子の分泌に影響を及ぼすのかを明らかにすることで、蠕動運動制御メカニズムの解明を目指しています。
【発表論文】
1) 黄体の機能調節機構の解明
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mechanisms of prostaglandin F2α stimulated by luteinizing hormone in the
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■Kawaguchi S, Sakumoto R, Okuda K: Induction of the expressions of antioxidant
enzymes by luteinizing hormone in the bovine corpus luteum. J Reprod Dev,
in press
■Kawaguchi S, Bowolaksono A, Sakumoto R, Okuda K: Luteoprotective roles
of luteinizing hormone are mediated by not only progesterone production
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Dev, in press
■Abe M, HojoT, Kozai K, Okuda K: Possible role of insulin-like factor
3 in the bovine corpus luteum. J Vet Med Sci, in press
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F2alpha and hydrogen peroxide in the regulation of Copper/Zinc superoxide
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■Yoshioka S, Acosta TJ, Okuda K: Roles of cytokines and progesterone in
the regulation of the nitric oxide generating system in bovine luteal endothelial
cells. Mol Reprod Dev, 79, 689-696 (2012)
■Kozai K, Hojo T, Takahashi M, Acosta TJ, Nambo Y, Okuda K: Seasonal changes
in luteal progesterone concentration and mRNA expressions of progesterone
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58 (4), 393-397 (2012)
■Shimizu T, Kaji A, Murayama C, Magata F, Shirasuna K, Wakamiya K, Okuda
K, Miyamoto A: Effects of interleukin-8 on estradiol and progesterone production
by bovine granulosa cells from large follicles and progesterone production
by luteinizing granulosa cells in culture. Cytokine, 57 (1), 175-181 (2012)
■Sakumoto R, Vermehren M, Kenngott RA, Okuda K, Sinowatz F: Localization
of gene and protein expressions of tumor necrosis factor-α (TNF), and TNF
receptor types I and II in the bovine corpus luteum during the estrous
cycle. J Anim Sci, 89 (10), 3040-3047 (2011)
■Lee SH, Acosta TJ, Nakagawa Y, Okuda K: Role of nitric oxide in the regulation
of superoxide dismutase and prostaglandin F2α production in bovine luteal
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the levels of progesterone receptor mRNA and protein in the bovine corpus
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■Hojo T, Al-zi'abi MO, Komiyama J, Manabe N, Acosta TJ, Okuda K: Expression
and localization of cFLIP, an anti-apoptotic factor, in the bovine corpus
luteum. J Reprod Dev, 56(2), 223-229 (2010)
■Nishimura R, Okuda K: Hypoxia is important for establishing vascularization
during corpus luteum formation in cattle. J Reprod Dev, 56 (1), 110-116
(2010)
■Skarzynski DJ, Okuda K: Inter- and intra-cellular mechanisms of prostaglandin
F2α action during corpus luteum regression in cattle.
Nottingham University Press,Volume 7, Number 1, 20, 303-322 (2010)
■Hojo T, Al-zi'abi MO, Skarzynski DJ, Acosta TJ, Okuda K: Changes in the
vasculature of bovine corpus luteum during the estrous cycle and prostaglandin
F2α-induced luteolysis.
J Reprod Dev, 55 (5), 512-517 (2009)
■Lee SH, Acosta TJ, Yoshioka S, Okuda K: Prostaglandin F2α regulates the
nitric oxide generating system in bovine luteal endothelial cells.
J Reprod Dev, 55 (4), 418-424 (2009)
■Taniguchi H, Komiyama J, Viger RV, Okuda K: The expression of the nuclear
receptors NR5A1 and NR5A2 and transcription factor GATA6 correlates with
steroidogenic gene expression in the bovine corpus luteum.
Mol Reprod Dev, 76 , 873-880 (2009)
■Acosta TJ, Bah MM, Korzekwa A, Woclawek-Potocka I, Markiewicz W, Jaroszewski
JJ, Okuda K, Skarzynski DJ: Acute changes in circulating concentrations
of progesterone, nitric oxide and partial pressure of oxygen during prostaglandin
F2α induced luteolysis in cattle. J Reprod Dev, 55 (2) 149-155 (2009)
■Al-zi'abi MO, Bowolaksono A, Okuda K: Survival role of locally produced
acetylcholine in the bovine corpus luteum. Biol Reprod, 80 (4), 823-832
(2009)
■ Acosta TJ, Bah MM, Korzekwa A, Woclawek-Potocka I, Markiewicz W, Jaroszewski
JJ, Okuda K, Skarzynski DJ Acute changes in circulating concentrations
of progesterone, nitric oxide and partial pressure of oxygen during prostaglandin
F2α induced luteolysis in cattle. J Reprod Dev, 55 (2) 149-155 (2009)
■ Skarzynski DJ, Piotrowska KK, Bah MM, Korzekwa A, Woclawek-Potocka I,
Sawai K, Okuda K Effects of exogenous tumour necrosis factor-α on the secretory
function of the bovine reproductive tract depend on tumour necrosis factor-α
concentrations. Reprod Domest Anim, 44, 371-379 (2009)
■ Skarzynski DJ, Ferreira-Dias G, Okuda K Regulation of luteal function
and corpus luteum regression in cows: hormonal control, immune mechanisms
and inter-cellular communication Reprod Domest Anim, 43(Suppl. 2), 57-65
(2008)
■ Nishimura R, Sakumoto R, Tatsukawa Y, Acosta TJ, Okuda K Oxygen concentration
is an important factor for modulating progesterone synthesis in bovine
corpus luteum. Endocrinology, 147(9): 4273-80 (2006)
■ Woclawek-Potocka I, Bober A, Korzekwa A, Okuda K, Skarzynski DJ Equol
and para-ethyl-phenol stimulate prostaglandins F2α in bovine corpus luteum.
Prostaglandins other Lipid Mediat, 79: 287-297 (2006)
■ Bah MM, Acosta T, Pilawski W, Deptula KM, Okuda K, Skarzynski DJ Role
of intraluteal prostaglandin F2α, progesterone and oxytocin in basal and
pulsatile progesterone release from developing bovine corpus luteum. Prostaglandins
other Lipid Mediat, 79: 218-229 (2006)
■ Shibaya M, Deptula KM, Korzekwa A, Okuda K, Skarzynski DJ Involvement
of the cytoskeleton in oxytocin secretion by cultured bovine luteal cells.
Biol Reprod, 72(1): 200-205 (2005)
■ Korzekwa A, Jaroszewski JJ, Bogacki M, Deptula KM, Maslanka TS, Acosta
TJ, Okuda K, Skarzynski DJ Effects of prostaglandin F2α and nitric oxide
on the secretory function of the bovine luteal cells. J Reprod Dev, 50(4):
411-417 (2004)
■ Nishimura R, Shibaya M, Skarzynski DJ, and Okuda K Progesterone stimulation
by LH involves the phospholipase-C pathway in bovine luteal cells. J Reprod
Dev, 50(2): 257-261 (2004)
■ Taniguchi H, Uenoyama Y, Miyamoto Y, Okuda K Intra-luteal lipoxygenase
products modulate progesterone production in cultured bovine luteal cells.
Prostaglandins Other Lipid Mediat, 67: 49-60 (2002)
■ Okuda K, Uenoyama Y, Berisha B, Lange IG, Taniguchi H, Kobayashi S,
Kobayashi SI, Miyamoto A, and Schams D Estradiol-17α is produced in bovine
corpus luteum. Biol. Reprod., 65(6): 1634-1639 (2001)
■ Okuda K, Skarzynski D Luteal prostaglandin F2α: new concepts of prostaglandin
F2α secretion and its actions within the bovine corpus luteum. Asia-Aust.
J Anim Sci, 13(3): 390-400 (2000)
■ Skarzynski D, Kobayashi S, Okuda K Influence of nitric oxide and noradrenaline
on prostaglandin F2α-induced oxytocin secretion and intracellular calcium
mobilisation in cultured bovine luteal cells. Biol Reprod, 63(4): 1000-1005
(2000)
■ Uenoyama Y, Schams D, Okuda K Basic fibroblast growth factor-induced
prostaglandin production and its intracellular mechanisms in cultured bovine
luteal cells. J Reprod Dev, 46(2): 93-99 (2000)
■ Skarzynski D, Okuda K Different actions of noradrenaline and nitric
oxide on the output of prostaglandins and progesterone in cultured bovine
luteal cells. Prostaglandins Other Lipid Mediat, 60(1): 35-47 (2000)
■ Skarzynski D, Okuda K Sensitivity of bovine corpora lutea to prostaglandin
F2α is dependent on progesterone, oxytocin and prostaglandins. Biol Reprod,
60(6): 1292-1298 (1999)
■ Okuda K, Uenoyama Y, Lee KW, Sakumoto R, and Skarzynski DJ Progesterone
stimulation by prostaglandin F2α involves protein kinase C pathway in cultured
bovine luteal cells. J Reprod Dev, 44(1): 79-84 (1998)
2) 黄体細胞におけるアポトーシス (プログラムされた細胞死) 調節機構の解明
■Sakumoto R, Vermehren M, Kenngott RA, Okuda K, Sinowatz F: Localization
of gene and protein expressions of tumor necrosis factor-α (TNF), and TNF
receptor types I and II in the bovine corpus luteum during the estrous
cycle. J Anim Sci, 89 (10), 3040-3047 (2011)
■Hojo T, Oda A, Lee SH, Acosta TJ, Okuda K: Effect of tumor necrosis factor
α (TNF) and interferon γ on the viability and mRNA expression of TNF receptor
type Ⅰ in the endothelial cells from bovine corpus luteum. J Reprod Dev,
56(5), 515-519 (2010)
■ Bowolaksono A, Nishimura R, Hojo T, Sakumoto R, Acosta TJ, Okuda K Anti-apoptotic
roles of prostaglandin E2 and F2α in bovine luteal steroidogenic cells.
Biol Reprod, 79(2), 310-317 (2008)
■ Nishimura R, Komiyama J, Tasaki Y, Acosta TJ, Okuda K Hypoxia promotes
luteal cell death in bovine corpus luteum. Biol Reprod, 78(3), 529-536
(2008)
■ Komiyama J, Nishimura R, Lee H-Y, Sakumoto R, Tetsuka M, Acosta T, Skarzynski
DJ, Okuda K Cortisol is a suppressor of apoptosis in bovine corpus luteum.
Biol Reprod, 78(5), 888-895 (2008)
■ Skarzynski DJ, Shibaya M, Tasaki Y, Korzekwa A, Murakami S, Woclawek-Potocka
I, Majewska M, Okuda K Fas-mediated apoptosis is suppressed by calf serum
in cultured bovine luteal cells. Reprod Biol, 7(1): 3-15 (2007)
■ Korzekwa A, Woclawek-Potocka I, Okuda K, Acosta TJ, Skarzynski DJ Nitric
oxide in bovine corpus luteum: possible mechanisms of action in luteolysis.
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■ Sugino N, Okuda K. Species-differences in the mechanism for apoptosis
during structural luteolysis. J Reprod Dev, 53(5): 977-986 (2007)
■ Korzekwa AJ, Okuda K, Woclawek-Potocka I, Murakami S, Skarzynski DJ
Nitric oxide induces apoptosis in bovine luteal cells. J Reprod Dev, 52(3):
353-361 (2006)
■ Tatsukawa Y, Bowolaksono A, Nishimura R, Komiyama J, Acosta TJ, Okuda
K. Possible roles of intracellular cyclic AMP, protein kinase C and calcium
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Woclawek-Potocka I, Skarzynski DJ Progesterone is a suppressor of apoptosis
in bovine luteal cells. Biol Reprod, 71(6): 2065-2071 (2004)
■ Taniguchi H, Yokomizo Y, Okuda K Fas/Fas ligand mediates luteal cell
death in bovine corpus luteum. Biol Reprod, 66(3): 754-759 (2002)
3) サイトカインが黄体機能および子宮内膜機能に及ぼす影響
■Okuda K, Sakumoto R, Okamoto N, Acosta TJ, Abe H, Okada H, Sinowatz F,
Skarzynski DJ: Cellular localization of genes and proteins for tumor necrosis
factor-α (TNF), TNF receptor types I and II in bovine endometrium. Mol
Cell Endocrinol, 330(1-2), 41-48 (2010)
■ Lee HY, Acosta TJ, Skarzynski DJ, Okuda K Prostaglandin F2α stimulates
11β-Hydroxysteroid Dehydrogenase 1 enzyme bioactivity and protein expression
in bovine endometrial stromal cells. Biol Reprod, 80 (4), 657-664 (2009)
■ Korzekwa A, Murakami S, Woclawek-Potocka I, Bah MM, Okuda K, Skarzynski
DJ The influence of tumor necrosis factor α (TNF) on the secretory function
of bovine corpus luteum: TNF and its receptors expression during the estrous
cycle. Reprod Biol, 8 (3), 245-262 (2008)
■ Tanikawa M, Lee HY, Watanabe K, Majewska M, Skarzynski DJ, Park S-B,
Lee D-S, Park C-K, Acosta TJ, Okuda K Regulation of prostaglandin biosynthesis
by interleukin-1 in cultured bovine endometrial cells. J Endocrinol, 199,
425-434 (2008)
■ Skarzynski DJ, Piotrowska KK, Bah MM, Korzekwa A, Woclawek-Potocka I,
Sawai K, Okuda K Effects of exogenous tumour necrosis factor-α on the secretory
function of the bovine reproductive tract depend on tumour necrosis factor-α
concentrations. Reprod Domest Anim, 44, 371-379 (2009)
■ Sakumoto R, Ito I, Okuda K Changes in expression of 11β-hydroxysteroid
dehydrogenase type-1, type-2 and glucocorticoid receptor mRNAs in porcine
corpus luteum during the estrous cycle. Mol Reprod Dev, 75(5), 925-930
(2008)
■ Skarzynski DJ, Ferreira-Dias G, Okuda K Regulation of luteal function
and corpus luteum regression in cows: hormonal control, immune mechanisms
and inter-cellular communication Reprod Domest Anim, 43(Suppl. 2), 57-65
(2008)
■ Skarzynski DJ, Woclawek-Potocka I, Korzekwa A, Bah MM, Piotrowska K,
Barszczewska B, Okuda K Infusion of exogenous tumor necrosis factor dose
dependently alters the length of the luteal phase in cattle: differential
response to treatment with indomethacin and L-NAME, a nitric oxide synthase
inhibitor. Biol Reprod, 76: 619-627 (2007)
■ Shibaya M, Matsuda A, Hojo T, Acosta TJ, Okuda K Expressions of estrogen
receptors in bovine corpus luteum: cyclic changes and effects of prostaglandin
F2α and cytokines. J Reprod Dev, 53(5): 1059-1068(2007)
■ Sakumoto R, Komatsu T, Kasuya E, Saito T, Okuda K Expression of mRNAs
for interleukin-4, interleukin-6 and their receptors in porcine corpus
luteum during the estrous cycle. Domest Anim Endocrinol, 31(3): 246-257
(2006)
■ Okuda K, Sakumoto R Regulation of uterine function by cytokines in cows.
Possible actions of tumor necrosis factor-a and interferon-t. Anim Sci
J: 77, 266-274 (2006)
■ Tanikawa M, Acosta TJ, Fukui T, Murakami S, Korzekwa A, Skarzynski DJ,
Piotrowska KK, Park C-K, Okuda K Regulation of prostaglandin synthesis
by interleukin-1α in bovine endometrium during the estrous cycle. Prostaglandins
Other Lipid Mediat, 78: 279-290 (2005)
■ Skarzynski DJ, Jaroszewski JJ, Okuda K Role of tumor necrosis factor-α
and nitric oxide in luteolysis in cattle. Domest Anim Endocrinol, 29: 340-346
(2005)
■ Nishimura R, Bowolaksono A, Acosta TJ, Murakami S, Piotrowska K, Skarzynski
DJ and Okuda K Possible role of interleukin-1 in the regulation of bovine
corpus luteum throughout the estrous cycle. Biol Reprod, 71(5): 1688-1693
(2004)
■ Okuda K, Kasahara Y, Murakami S, Takahashi H, Woclawek-Potocka I, and
Skarzynski DJ Interferon-τ blocks the stimulatory effect of tumor necrosis
factor-á on prostaglandin F2α synthesis by bovine endometrial stromal cells.
Biol Reprod, 70(1): 191-197 (2004)
■ Sakumoto R, and Okuda K Possible actions of tumor necrosis factor-α
in ovarian function. J Reprod Dev, 50(1): 39-46 (2004)
■ Sakumoto R, Shibaya M, and Okuda K Tumor necrosis factor-α (TNF) inhibits
progesterone and estradiol-17α production from cultured granulosa cells:
Presence of TNF receptors in bovine granulosa and theca cells. J Reprod
Dev, 49(6): 441-449 (2003)
■ Okuda K, and Sakumoto R Multiple roles of TNF super family members in
corpus luteum function. Reproductive Biology and Endocrinology, 1, 95 (2003).
http://www.rbej.com/content/1/1/95
■ Skarzynski DJ, Bah MM, Deptula KM, Woclawek-Potocka I, Korzekwa A, Pilawski
W, and Okuda K Tumor necrosis factor-α regulates prostaglandin production
and the length of the estrous cycle in conscious cattle. Biol Reprod, 69(12):
1907-1913 (2003)
■ Murakami S, Miyamoto Y, Skarzynski D, Okuda K Effects of tumor necrosis
factor-α on secretion of prostaglandin E2 and F2α in bovine endometrium
throughout the estrous cycle. Theriogenology, 55(8): 1667-1678 (2001)
■ Sakumoto R, Murakami S, Okuda K Tumor necrosis factor-α stimulates prostaglandin
F2α secretion by bovine luteal cells via activation of mitogen-activated
protein kinase and phospholipase A2 pathways. Mol Reprod Dev, 56(3): 387-391
(2000)
■ Skarzynski D, Miyamoto Y, Okuda K Production of prostaglandin F2α by
cultured bovine endometrial cells in response to tumor necrosis factor-α:
cell type specificity and intracellular mechanisms. Biol Reprod, 62(5):
1116-1120 (2000)
■ Miyamoto Y, Skarzynski D, Okuda K Is tumor necrosis factor-α trigger
for the initiation of endometrial prostaglandin F2α release at luteolysis
in cattle. Biol Reprod, 62(5): 1109-1115 (2000)
■ Sakumoto R, Berisha B, Kawate N, Schams D, Okuda K Tumor necrosis factor-α
and its receptor in bovine corpus luteum throughout the estrous cycle.
Biol Reprod, 62(1): 192-199 (2000)
■ Sakumoto R, Kishi H, Iga K, Okano A, Okuda K Tumor necrosis factor-α
and its receptor in the corpus luteum of pregnant cows. Mol Reprod Dev,
55(3): 406-411 (2000)
4) 子宮内膜のプロスタングランディン分泌調節因子の検索
■Duong H, Skarzynski D, Piotrowska-Tomala KK, Bah M, Jankowska P, Lukasik
K, Okuda K, Acosta TJ: Conversion of cortisone to cortisol and prostaglandin
F2α production by the reproductive tract of cows at the late luteal stage
in vivo. Reprod Domest Anim, 47, 939-945 (2012)
■Tasaki Y, Nishimura R, Shibaya M, Lee HY, Acosta TJ, Okuda K: Expression
of VEGF and its receptors in the bovine endometrium throughout the estrous
cycle: effects of VEGF on prostaglandin production in endometrial cells.
J Reprod Dev, 56 (2), 230-235 (2010)
■ Lee HY, Acosta TJ, Skarzynski DJ, Okuda K Prostaglandin F2α stimulates
11β-Hydroxysteroid Dehydrogenase 1 enzyme bioactivity and protein expression
in bovine endometrial stromal cells. Biol Reprod, 80 (4), 657-664 (2009)
■ Woclawek-Potacka I, Komiyama J, Saulnier-Blache J, Brzezicka E, Bah
M, Okuda K, Skarzynski D Lysophosphatic acid modulates prostaglandin secretion
in the bovine uterus. Reproduction, 137 (1), 95-105 (2009)
■ Tanikawa M, Lee HY, Watanabe K, Majewska M, Skarzynski DJ, Park S-B,
Lee D-S, Park C-K, Acosta TJ, Okuda K Regulation of prostaglandin biosynthesis
by interleukin-1 in cultured bovine endometrial cells. J Endocrinol, 199,
425-434 (2008)
■ Skarzynski DJ, Piotrowska KK, Bah MM, Korzekwa A, Woclawek-Potocka I,
Sawai K, Okuda K Effects of exogenous tumour necrosis factor-α on the secretory
function of the bovine reproductive tract depend on tumour necrosis factor-α
concentrations. Reprod Domest Anim, 44, 371-379 (2009)
■ Lee HY, Acosta TJ, Tanikawa M, Sakumoto R, Kom iyama J, Tasaki Y, Piskula
M, Skarzynski DJ, Tetsuka M, Okuda K. The role of glucocorticoid in the
regulation of prostaglandin biosynthesis in non-pregnant bovine endometr
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(2007)
■ Woclawek-Potocka I, Borkowski K, Korzekwa A, Okuda K, Skarzynski DJ
Phyto- and endogenous estrogens differently activate intracellular calcium
ion mobilization in the bovine endometrial cells. J Reprod Dev, 52(6):
731-740 (2006)
■ Woclawek-Potocka I, Okuda K, Acosta TJ, Korzekwa A, Pilawaski W, Skarzynski
DJ Phytoestrogen metabolites are much more active than phytoestrogens themselves
in increasing prostaglandin F2α synthesis via prostaglndins F2α synthase-like
2 stimulation in bovine endometrium. Prostaglandins Other Lipid Mediat,
78: 202-217 (2005)
■ Woclawek-Potocka I, Acosta TJ, Korzekwa A, Bah MM, Shibaya M, Okuda
K, Skarzynski DJ Phytoestrogens modulate prostaglandin production in bovine
endometrium: cell type specifity and intracellular mechanisms. Exp Biol
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■ Okuda K, Miyamoto Y, and Skarzynski D Regulation of endometrial prostaglandin
F2α synthesis during luteolysis and early pregnancy in cattle. Dom Anim
Endocrinol, 23: 255-264 (2002)
■ Skarzynski D, Uenoyama Y, Kotwica J, Okuda K Noradrenaline stimulates
the production of prostaglandin F2α in cultured bovine endometrial cells.
Biol Reprod, 60(2): 277-282 (1999)
■ Miyamoto Y, Skarzynski DJ, Uenoyama Y, and Okuda K Secretion of prostaglandin
E2 and F2α by the bovine endometrium in response to oxytocin during the
estrous cycle. J Reprod Dev, 44(3): 289-295 (1998)
5) 卵管平滑筋運動に関わる因子の分泌調節機構の解明
■Szostek AZ, Siemieniuch MJ, Deptula K, Woclawek-Potocka I, Majewska M,
Okuda K, Skarzynski DJ: Ovarian steroids modulate tumor necrosis factor-α
and nitric oxide- regulated prostaglandin secretion by cultured bovine
oviductal epithelial cells. Domest Anim Endocrinol, 41, 14-23 (2011)
■Siemieniuch M, Woclaweak-Potocka I, Deptula K, Okuda K, Skarzynski DJ:
Effects of tumor necrosis factor-α and nitric oxide on prostaglandins secretion
by the bovine oviduct differ in the isthmus and ampulla and depend on the
phase of the estrous cycle. Exp Biol Med, 234 (9), 1056-1066 (2009)