Chemistry of Bioactive Compounds
Search and Production of Useful Bioactive Compounds
Staff
Research Topics
Studies on novel enzymes useful for synthesizing or searching for anticancer compounds Microbial enzyme-catalyzed high functionalization of olive constituents Microbial synthesis or degradation of functional glycosides High functionalization of woody biomass using microbial transformation Search for specific inhibitors of insect enzymes Structure-activity relationship for novel chitinolytic enzyme inhibitors Search for compounds with insect growth regulating activity
Search and use of new enzymes effective for production of useful substances
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(1) We can synthesize various dehydrogenated cyclic dipeptides by using the novel cyclic dipeptide oxidase that we found in actinomycetes. We are investigating various properties of this unique enzyme and conducting applied research on highly sensitive detection of cyclic dipeptide and production of dehydrogenated cyclic dipeptide using this enzyme. (2) We are preparing new antioxidant active substances by enzymatic conversion from olive secondary metabolites, and are studying their usage. (3) We are conducting a detailed study of microbial enzymes that show characteristic transglycosylation activity. (4) We are studying methods for treating woody biomass with |
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Search for chitinolytic enzyme inhibitors and analysis of their bioactivity
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The epidermis of insects and the cell wall of filamentous fungi contain the polysaccharide chitin. Since the metabolism of chitin is essential for the growth of insects and filamentous fungi, the enzymes responsible for the metabolism of chitinase and β-N-acetylglucosaminidase are expected to be applied to pest control agents and antibacterial agents. We have conducted research to find inhibitors of these enzymes in microbial metabolites. We have discovered two novel β-N-acetylglucosaminidase (GlcNAcase) inhibitors with distinct specificity. We are trying to elucidate the molecular basis of their inhibitory specificity and to search further novel GlcNAcase inhibitors. |
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Publication List
- Hasanah, U., Miki, K., Nitoda, T., Kanzaki H.: Aerobic bioconversion of C-glycoside mangiferin into its aglycone norathyriol by an isolated mouse intestinal bacterium. Bioscience, Biotechnology, and Biochemistry, 85(4), 989–997 (2021)
- Mushiake,Y., Tsuchida, A., Yamada, A., Kanzaki, H., Okuda, T., Nitoda, T.: New analogs of pochonicine, a potent β-N-acetylglucosaminidase inhibitor from fungus Pochonia suchlasporia var. suchlasporiaTAMA 87. Journal of Pesticide Science, 46(1), 115-119 (2021)
- Mushiake,Y., Tsuchida, A., Yamada, A., Kanzaki, H., Okuda, T., Nitoda, T.: New analogs of pochonicine, a potent β-N-acetylglucosaminidase inhibitor from fungus Pochonia suchlasporia var. suchlasporiaTAMA 87. Journal of Pesticide Science, 46(1), 115-119 (2021)
- Suminto, T., Takatsuji E., Iguchi A.,; Kanzaki H., Okuda T., Nitoda, T.: A new asteltoxin analog with insecticidal activity from Pochonia suchlasporia TAMA 87. Journal of Pesticide Science, 45(2), 81-85 (2020)
- Chumpol, S., Kantachote, D., Rattanachuay, P., Torpee, S., Nitoda, T., Kanzaki, H.: Optimization of culture conditions for production of antivibrio compounds from probiotic purple nonsulfur bacteria against acute hepatopancreatic necrosis disease-causing Vibrio parahaemolyticus and Vibrio spp. Aquaculture, 505, 72-83 (2019),
- Sohsomboon, N., Kanzaki, H., Nitoda, T.: Unique antimicrobial spectrum of ophiobolin K produced by Aspergillus ustus. Bioscience, Biotechnology, and Biochemistry, 82(3), 422-424 (2018).
- Chumpol, S., Kantachote, D., Nitoda, T., Kanzaki, H.: Administration of purple nonsulfur bacteria as single cell protein by mixing with shrimp feed to enhance growth, immune response and survival in white shrimp (Litopenaeus vannamei) cultivation. Aquaculture, 489, 85-95 (2018).
- Arunrattiyakorn, P., Kuno, M., Aree, T., Laphookhieo, S., Sriyatep, T., Kanzaki, H.: Biotransformation of β-Mangostin by an Endophytic Fungus of Garcinia mangostana to Furnish Xanthenes with an Unprecedented Heterocyclic Skeleton. Journal of Natural Products (2018), 81(10), 2244-2250
- Arunrattiyakorn, P., Kuno, M., Aree, T., Laphookhieo, S., Sriyatep, T., Kanzaki, H.: Biotransformation of β-Mangostin by an Endophytic Fungus of Garcinia mangostana to Furnish Xanthenes with an Unprecedented Heterocyclic Skeleton. Journal of Natural Products (2018), 81(10), 2244-2250
- Shiota, H., Kanzaki, H., Hatanaka, T., Nitoda, T.: TMG-chitotriomycin as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases. Carbohydrate Research, 375, 29-34 (2013)