岡山大学大学院ヘルスシステム統合科学研究科／工学部化学生命系学科

論文・雑誌記事

2023

Hakim TFN, Watanabe K, Fujimoto S, Kitamatsu M, Ohtsuki T, Self-assembly of peptide amphiphiles with alkyl groups for siRNA delivery. Chem. Lett., 52, 815-818 [doi]

Ikawa Y, Wakai T, Funahashi H, Soe TH, Watanabe K, Ohtsuki T, Photo-dependent cytosolic delivery of shRNA into a single blastomere in a mouse embryo. Scientific Reports, 13, 13050 [doi]

Thu M, Yanai K, Shigeto H, Yamamura S, Watanabe K, Ohtsuki T, FRET probe for detecting two mutations in one EGFR mRNA. Analyst, 148, 2626–2632 [doi]

Hakata Y, Yamashita K, Hashimoto S, Ohtsuki T, Miyazawa M, Kitamatsu M, Adjusting heterodimeric coiled-coils (K/E zipper) to connect autophagy-inducing peptide with cell-penetrating peptide. Pharmaceutics, 15, 1048 [doi]

坂東晃成、渡邉和則、大槻高史、Photochemical internalizationに基づく光依存的細胞質内RNA導入法の副作用低減. 日本レーザー医学会誌, 44 (1), 62-68 [doi]

2022

Fithroni AB, Kobayashi K, Uji H, Ishimoto M, Akehi M, Ohtsuki T, Matsuura E, Novel self-forming nanosized DDS particles for BNCT: utilizing a hydrophobic boron-cluster and its molecular glue effect. Cells, 11, 3307 [doi]

Sumi N, Nagahiro S, Nakata E, Watanabe K, Ohtsuki T, Ultrasound-dependent RNAi using TatU1A-rose bengal conjugate. Bioorg. Med. Chem. Lett., 68, 128767 [doi]

Ishii K, Tsuchitani S, Toyama M, Shigeto H, Yamamura S, Ohtsuki T, Imai Y, Kitamatsu M, Fluorescence ratiometric DNA detection by peptide nucleic acid-pyrene binary probes. Bioorg. Med. Chem. Lett., 71, 128838 [doi]

Shigeto H, Kishi T, Ishii K, Ohtsuki T, Yamamura S, Kitamatsu M, Tuning a structure of peptide nucleic acid molecular beacon and DNA detection by the hybrid with quencher-modified DNA. Processes, 10, 722 [doi]

渡邉 和則，大槻 高史，光応答的なRNA デリバリー, Drug Delivery System（特集：mRNA医薬・mRNAワクチンの新展開）37 (3), 229-236 [doi]

2021

Tabara K, Watanabe K, Shigeto H, Yamamura S, Kishi T, Kitamatsu M, Ohtsuki T, Fluorophore–PNA–quencher/quencher–DNA probe for miRNA detection. Bioorg. Med. Chem. Lett., 51, 128359 [doi]

Watanabe K, Nawachi T, Okutani R, Ohtsuki T, Photocontrolled apoptosis induction using precursor miR-664a and an RNA carrier-conjugated with photosensitizer. Scientific Reports, 11, 14936 [doi]

Watanabe K, Ohtsuki T, Inhibition of HSF1 and SAFB granule formation enhances apoptosis induced by heat stress. Int. J. Mol. Sci., 22, 4982 [doi]

Zhou S, Watanabe K, Koide S, Kitamatsu M, Ohtsuki T, Minimization of apoptosis-inducing CPP-Bim peptide. Bioorg. Med. Chem. Lett., 36, 127811 [doi]

Kitamatsu M, Yuasa H, Ohtsuki T, Michiue H, Complementary leucine zippering system for effective intracellular delivery of proteins by cell-penetrating peptides. Bioorg. Med. Chem. 33, 116036 [doi]

Lim M.S.H, Nishiyama Y, Ohtsuki T, Watanabe K, Kobuchi H, Kobayashi K, Matsuura E, Lactosome-conjugated siRNA nanoparticles for photo-enhanced gene silencing in cancer cells. J. Pharm. Sci. 119, 1788-1798 [doi]

Hirata R, Hirakawa K, Shimada N, Watanabe K, Ohtsuki T, Fluorescence lifetime probes for detection of RNA degradation. Analyst, 146, 277-282 [doi]

Lim M.S.H, Ohtsuki T, Takenaka F, Kobayashi K, Akehi M, Uji H, Kobuchi H, Sasaki T, Ozeki E, Matsuura E, [REVIEW] A novel ⁸⁹Zr-labeled DDS device utilizing human IgG variant (scFv): “lactosome” nanoparticle-based theranostics for PET imaging and targeted therapy. Life, 11, 158 [doi]

Soe T.H, Watanabe K, Ohtsuki T, [REVIEW] Photoinduced endosomal escape mechanism: a view from photochemical internalization mediated by CPP-photosensitizer conjugates. Molecules, 26, 36 [doi]

Endoh T, Rozners E, Ohtsuki T, [EDITORIAL] Nucleic acids chemistry and engineering: Special issue on nucleic acid conjugates for biotechnological applications. Applied Sciences, 11, 3594 [doi]

2020

Kim H, Watanabe S, Kitamatsu M, Watanabe K, Ohtsuki T, Cell cycle dependence of apoptosis photo-triggered using peptide-photosensitizer conjugate. Scientific Reports, 10, 19087 [doi]

Shigeto H, Yamada E, Kitamatsu M, Ohtsuki T, Iizuka A, Akiyama Y, Yamamura S, Analysis of single nucleotide-mutated single-cancer cells using the combined technologies of single-cell microarray chips and peptide nucleic acid-DNA probes. Micromachines, 11, 628 [doi]

Hakata Y, Ishikawa S, Ohtsuki T, Miyazawa M, Kitamatsu M, Intracellular delivery of a peptide nucleic acid-based hybrid of an autophagy-inducing peptide with a cell-penetrating peptide. Organic & Biomolecular Chemistry, 18, 1978–1986 [doi]

Miyoshi Y, Kadono M, Okazaki S, Nishimura A, Kitamatsu M, Watanabe K, Ohtsuki T, Endosomal escape of peptide-photosensitizer conjugates is affected by amino acid sequences near the photosensitizer. Bioconjugate Chemistry, 31, 916−922 [doi]

渡邉 和則，大槻 高史，RNAキャリア・光応答的RNAデリバリー, バイオマテリアル（特集：核酸創薬に貢献するバイオマテリアル）38 (2), 118-123

2019

Soe T.H, Nanjo T, Watanabe K, Ohtsuki T, Relation of photochemical internalization to heat, pH, and Ca²⁺ ions. Photochemistry and Photobiology, 95, 1395–1402 [doi]

Shigeto H, Ohtsuki T, Iizuka A, Akiyama Y, Yamamura S, Imaging analysis of EGFR mutated cancer cells using peptide nucleic acid (PNA) – DNA probes. Analyst, 144, 4613-21 [doi]

Kim H, Kitamatsu M, Ohtsuki T, Combined apoptotic effects of peptide and miRNA in a peptide/miRNA nanocomplex. J. Biosci. Bioeng. 29, 878–881 [doi]

Miyoshi Y, Ohtsuki T, Kashida H, Asanuma H, Watanabe K, In-stem molecular beacon targeted to a 5'-region of tRNA inclusive of the D arm that detects mature tRNA with high sensitivity. PLOS ONE, 14, e0211505 [doi]

Hakata Y, Michiue H, Ohtsuki T, Miyazawa M, Kitamatsu M, A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus. Bioorg. Med. Chem. Lett. 29, 878–881 [doi]

2018

Shiraga K, Soe T. H, Matsumoto S, Watanabe K, Ohtsuki T, Red and near-infrared light-directed cytosolic delivery of two different RNAs using photosensitive RNA carriers. Bioconjugate Chemistry, 29, 3174-3179 [doi]

Watanabe K, Yamaji R, Ohtsuki T, MicroRNA-664a-5p promotes neuronal differentiation of SH-SY5Y cells. Genes to Cells, 23, 225–233 [doi]

Kim H, Kitamatsu M, Ohtsuki T, Enhanced intracellular peptide delivery by multivalent cell-penetrating peptide with bioreducible linkage. Bioorg. Med. Chem. Lett. 28, 378-381 [doi]

Miyoshi Y, Watanabe K, Formation mechanism and cellular functions of nuclear stress bodies induced by heat stress. Thermal Medicine, 34, 23-34 [doi]

竹中 文章，小林 和子，木村 俊作，小関 英一，大槻 高史，小渕 浩嗣，松浦 栄次，⁸⁹Zr標識ヒト抗体バリアントと新規DDSキャリアによるTheranostics技術, Drug Delivery System, 33 (3), 214-222 [doi]

渡邉 和則，大槻 高史，創薬を支える光技術，月刊光アライアンス, 6月号, pp.1-4

2017

Inaba Y, Watanabe K, Kitamatsu M, Nakata E, Harada A, Ohtsuki T, Ultrasound-dependent cytoplasmic internalization of a peptide-sonosensitizer conjugate. Bioorganic and Medicinal Chemistry, 25, 4212-4217 [doi]

Li J, Xu C, Shimada N, Miyoshi Y, Watanabe K, Cong W, Ohtsuki T, Detection of small, highly structured RNAs by molecular beacons. Analytical Methods, 9, 2971-2976 [doi]

Sato A, Suematsu T, Aihara K, Kita K, Suzuki T, Watanabe K, Ohtsuki T, Watanabe Y, Duplication of Drosophila melanogaster mitochondrial EF-Tu: pre-adaptation to T-arm truncation and exclusion of bulky aminoacyl residues. Biochemical Journal 474, 957–969 [doi]

赤星彰也，大槻高史，拡張翻訳系におけるケージド化合物の利用，化学，Vol. 72 (2) pp.64-65

2016

Ohtsuki T, Kanzaki S, Nishimura S, Kunihiro Y, Sisido M, Watanabe K, Phototriggered protein syntheses by using (7-diethylaminocoumarin-4-yl)methoxycarbonyl-caged aminoacyl tRNAs. Nature Communications, 7, 12501 [doi]

Watanabe K, Fujiwara, H, Kitamatsu M, Ohtsuki T, Photoinduced apoptosis using a peptide carrying a photosensitizer. Bioorg. Med.Chem. Lett. 26, 3115-3118 [doi]

Hori H, Terui Y, Nakamoto C, Iwashita C, Ochi A, Watanabe K, Oshima T, Effects of polyamines from Thermus thermophilus, an extreme-thermophilic eubacterium, on tRNA methylation by tRNA (Gm18) methyltransferase (TrmH). J. Biochem. 159, 509-517 [doi]

Watanabe K, Ohtsuki T, Photocontrolled intracellular RNA delivery using nanoparticles or carrier–photosensitizer conjugates. Prog. Mol. Biol. Transl. Sci. 39, 101-119 [doi]

Akahoshi, A, Matuura E, Ozeki E, Matsui H, Watanabe K, Ohtsuki T, Enhanced cellular uptake of lactosomes using cell-penetrating peptides. Sci. Technol. Adv. Mater. 17, 245-252 [doi]

大槻高史，細胞内のタンパク質合成を光でコントロールする！ Academist Journal 12月5日，研究コラム [web]

2015

Ohtsuki T, Miki S, Kobayashi S, Haraguchi T, Nakata E, Hirakawa K, Sumita K, Watanabe K, Okazaki S. The molecular mechanism of photochemical internalization of cell penetrating peptide-cargo-photosensitizer conjugates. Scientific Reports, 5, 18577 [doi]

Hakata Y. Tsuchiya S, Michiue H, Ohtsuki T, Matsui H, Miyazawa M, Kitamatsu M, Intracellular delivery of a peptide cargo by a cell-penetrating peptide via leucine-zippers does not affect the function of cargo, Chem. Commun. 51, 413-416 [doi]

大槻高史，光化学的に細胞質内に侵入するペプチド分子の設計，月刊 化学工業，7月号，34-39

2014

Akahoshi A, Doi Y, Sisido M, Watanabe K, Ohtsuki T, Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA. Bioorg. Med. Chem. Lett. 24, 5369–5372 [doi]

Watanabe K, Ijiri K, Ohtsuki T, mTOR regulates the nucleoplasmic diffusion of Xrn2 under conditions of heat stress. FEBS Lett, 588, 3454–3460 [doi]

Watanabe Y, Suematsu T, Ohtsuki T, Losing the stem-loop structure from metazoan mitochondrial tRNAs and co-evolution of interacting factors. Front. Genet. 5, 109 [doi]

Watanabe K, Ohtsuki T, [REVIEW] Intracellular delivery of RNA via RNA-binding proteins or peptides. Fundamental Biomedical Technologies, 7, 403-416

大槻高史，PCDR法とCLIP-RNAi法，生命化学研究レター，No.46，pp.10-14 [pdf]

2013年以前の代表的な論文

Matsushita-Ishiodori Y, Morinaga M, Watanabe K, Ohtsuki T, Near-infrared light-directed RNAi using a photosensitive carrier molecule. Bioconjugate Chemistry, 24, 1669–1673 (2013) [doi]

Watanabe K, Miyagawa R, Tomikawa C, Mizuno R, Takahashi A, Hori H, Ijiri K, Degradation of initiator tRNA^Met by Xrn1/2 via its accumulation in the nucleus of heat-treated HeLa cells. Nucleic Acids Res. 41, 4671-4685 (2013) [doi]

Matsushita-Ishiodori Y, Kuwabara R, Sakakoshi H, Endoh T, Ohtsuki T, Photosensitizing carrier proteins for photoinducible RNA interference. Bioconjugate Chemistry, 22, 2222–2226 (2011) [doi]

Endoh T, Sisido M, Ohtsuki T, Spatial regulation of specific gene expression through photoactivation of RNAi. Journal of Controlled Release, 137, 241–245 (2009) [doi]

Endoh T, Sisido M, Ohtsuki T, Cellular siRNA delivery mediated by a cell-permeant RNA-binding protein and photoinduced RNA interference. Bioconjugate Chemistry, 19, 1017–1024 (2008) [doi]

Doi Y, Ohtsuki T, Shimizu Y, Ueda T, Sisido M, EF-Tu mutants expand amino acid tolerance of protein biosynthesis system, J. Am. Chem. Soc. 129, 14458-14462 (2007) [doi]

Watanabe K, Toh Y, Suto K, Shimizu Y, Oka N, Wada T, Tomita K, Protein-based peptide-bond formation by aminoacyl-tRNA protein transferase. Nature, 449, 867-871 (2007) [doi]

Arita M, Suematsu T, Osanai A, Inaba T, Kamiya H, Kita K, Sisido M, Watanabe Y, Ohtsuki T. An evolutionary ‘intermediate state’ of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu. Nucleic Acids Res. 34, 5291-5299 (2006) [doi]

Suematsu T, Sato A, Sakurai M, Watanabe K, Ohtsuki T. A unique tRNA recognition mechanism of Caenorhabditis elegans mitochondrial EF-Tu2. Nucleic Acids Research, 33, 4683-4691 (2005) [doi]

Sakurai M, Ohtsuki T, Watanabe K. Modification at position 9 with 1-methyladenosine is crucial for structure and function of nematode mitochondrial tRNAs lacking the entire T-arm. Nucleic Acids Research, 33, 1653-1661 (2005) [doi]

Ohtsuki T, Sato A, Watanabe Y, Watanabe K, A unique serine-specific elongation factor Tu found in nematode mitochondria. Nature structural Biology, 9, 669-673 (2002) [doi]

Ohtsuki T, Sakurai M, Sato A, Watanabe K, Characterization of the interaction between the nucleotide exchange factor EF-Ts from nematode mitochondria and elongation factor Tu. Nucleic Acids Research, 30, 5444-5451 (2002) [doi]

Hirao I, Ohtsuki T, Fujiwara T, Mitsui T, Yokogawa T, Okuni T, Nakayama H, Takio K, Yabuki T, Kigawa T, Kodama K, Yokogawa T, Nishikawa K, Yokoyama S, An unnatural base pair for incorporating amino acid analogs into proteins. Nature Biotechnology, 20, 177-182 (2002) [doi]

Ohtsuki T, Watanabe Y, Takemoto, C, Kawai, G, Ueda, T, Kita, K, Kojima, S, Kaziro, Y, Nyborg, J, Watanabe K, An "elongated" translation elongation factor Tu for truncated tRNAs in nematode mitochondria. J. Biol. Chem. 276, 21571-21577 (2001) [doi]

Ohtsuki T, Kimoto M, Ishikawa M, Mitsui T, Hirao I, Yokoyama, S. Unnatural base pairs for specific transcription. Proc. Natl. Acad. Sci. USA. 98, 4922-4925 (2001) [doi]

著書

宇治広隆，大槻高史，松浦栄次，高分子ナノミセル・ペプチド-RNA ナノ複合体を基盤としたキャリア作製 // 北松瑞生，石井康稀，博多義之，宮澤正顯，道上宏之，大槻高史，ペプチドジッパー法による細胞膜透過ペプチドを用いたカーゴの細胞内デリバリー in「新規モダリティ医薬品のための新しいＤＤＳ技術と製剤化」技術情報協会 (2023) ISBN:978-4-86104-936-1

渡邉 和則，大槻 高史，RNAデリバリー in「核酸科学ハンドブック」 日本核酸化学会(監修)杉本 直己(編)，講談社サイエンティフク (2020) ISBN:978-4065207864

渡邉和則，大槻高史，非天然アミノ酸による生体反応の制御法 in「CSJ current review: 生体分子反応を制御する」 日本化学会編，化学同人 (2020) ISBN:978-4759813968

北松瑞生，博多義之，宮澤正顯，道上宏之，大槻高史，ペプチドファスナー法による生理活性ペプチドの細胞内デリバリー in「ペプチド創薬の最前線」木曽良明監修、シーエムシー出版 (2019) ISBN:978-4781314174

北松瑞生，道上宏之，大槻高史，光照射によりがん細胞を特異的に死滅させるペプチドシステムに関する研究 in「ペプチド医薬品のスクリーニング・安定化・製剤化技術」技術情報協会 (2017) ISBN:978-4861046872

Watanabe K, Ohtsuki T, Photocontrolled intracellular RNA delivery using nanoparticles or carrier–photosensitizer conjugates. in “Nanotechnology Tools for the Study of RNA” (Yoshizawa S. eds) Academic Press (2016) ISBN:978-0128045657

Watanabe K, Ohtsuki T, Intracellular delivery of RNA via RNA-binding proteins or peptides. in “Intracellular Delivery II” (Prokop A, Iwasaki Y, Harada A. eds) Springer (2014) ISBN:978-9401788953

Endoh T, Ohtsuki T, Cellular siRNA delivery using TatU1A and photo-induced RNA interference. in “RNA Interference: From Biology to Clinical Applications” Humana Press (2010) ISBN:978-1493961344

大槻高史，北松瑞生，人工核酸によるRNAの検出と精製 in「シングルセル解析の最前線」神原秀記・松永是・植田充美監修、シーエムシー出版 (2010) ISBN:978-4781302034

Ohtsuki T, Sisido M, The central dogma: from DNA to RNA, and to protein. in “Automation in Proteomics and Genomics” (Alterovitz G. eds) Wiley (2009) ISBN:978-0470727232

宍戸昌彦，大槻高史，「化学の指針シリーズ 生物有機化学」 全204頁，裳華房 (2008) ISBN:978-4785332204

Suzuki T, Ohtsuki T, Watanabe Y, Terasaki M, Hanada T, Watanabe, K, Structural and functional compensation by proteins for the RNA deficit of animal mitochondrial translation system. in “Cell-free translation system” (Spirin A. eds) Springer (2002) ISBN:978-3642639562

大槻高史，化学試薬による二次構造マッピング in「基礎生化学実験法 第４巻」，東京化学同人 (2000) ISBN:978-4807911837