Identification of gene Vrn-D4 for enabling wheat to adapt to areas with warm winters
Wheat is widely cultivated under diverse climatic conditions around the world. Vernalization plays an important role for avoiding injury from cold in winter, through suppressing precocious spike development before or during winter. In contrast, vernalization negatively affects adaptation in places with warm winter climates, and gene mutation so that vernalization is not needed could benefit wheat cultivation in such areas. Four genes, Vrn-1, Vrn-2, Vrn-3, and Vrn-4, are known to control vernalization in wheat, but until recently only the first three genes had been identified.
Kenji Kato and colleagues at the University of California, Davis, USA, identified the fourth vernalization gene Vrn-D4 located on the 5DS chromosome. Vrn-D4 proved to be the copy of gene Vrn-A1 on chromosome 5AL, which was duplicated and inserted into 5DS. Comparisons with Vrn-A1, revealed nucleotide substitution in exon 4 (A367C) and introns. Among them, two SNPs (Single Nucleotide Polymorphisms) in intron 1 were within the RIP-3 (RNA immunoprecipitation-3) motif and caused no requirement of vernalization through the interaction with TaGRP2.
Vrn-D4 was mostly found in common wheat (T. aestivum) of South Asian origin. All accessions of T. sphaerococcum endemic to Pakistan and India proved to have Vrn-D4 by which they don’t need vernalization, and successfully adapted to warm conditions in South Asia. It was therefore suggested that Vrn-D4 is closely related with the origin of T. sphaerococcum, which is specifically adapted to areas with warm winter climates.
Now, all of the four vernalization genes have been identified, providing important information for understanding vernalization in wheat and winter cereals. This finding significantly contributed to the breeding of new wheat cultivars with stable production, even under changing climates, by fine-tuning the flowering time.
・Authors: Nestor Kippes, Juan M. Debernardi, Hans A. Vasquez-Gross, Bala A. Akpinar, Hikment Budak, Kenji Kato, Shiaoman Chao, Eduard Akhunov, and Jorge Dubcovsky
・Title of original paper: Identification of the VERNALIZATION 4 gene reveals the origin of spring growth habit in ancient wheats from South Asia
・Journal, volume, pages and year: Proceedings of the National Academy of Sciences of the United States of America 112, E5401–E5410 (2015).
・Digital Object Identifier (DOI): 10.1073/pnas.1514883112
・Journal website: http://www.pnas.org/content/112/39/E5401.abstract
・Affiliations: Graduate School of Environmental and Life Science, Okayama University
・Department website: http://www.okayama-u.ac.jp/user/agr/eng/course_aps/plantgb.html