An hourglass model for molecular mechanism to determine pupation timing. At the early prepupal stage, the ftz-f1 gene is repressed by Blimp-1 during high levels of 20E. The shade gene is expressed independent of βFTZ-F1 and disappears by mid-prepupal stage. After the decline of 20E levels, Blimp-1 and its mRNA degrade rapidly (Agawa et al., 2007; Akagi and Ueda, 2011) allowing expression of βFTZ-F1 which induces Shade in the fat body at late prepupal period. Then Shade converts released E to 20E, inducing pupation.
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Research Highlights

Molecular “sands of time” identified for fruitfly development

Okayama University have identified genes and processes responsible for pupation timing in the development of fruit fly larvae into adult insects.

The timing mechanism between specific developmental stages such as pupation in insects is so far little understood. In the Drosophila melanogaster – or common fruit fly - it is known that a pulse of the molting hormone ecdysone (E) is released to initiate the transition of the larva into a “prepupa” and a second pulse 10 hours later is then thought to induce pupation at around 12 hours. Using knock down models and forced expression of normal or modified proteins Hiroshi Ueda and colleagues at Okayama University investigated the role of different proteins on the timing of this 12 hour interval.

Shade, a protein mainly expressed in an insect organ that stores fat and is also known to sense nutrition – “fat body” - converts the hormone E into its active form 20-hydroxyecdysone (20E). Ueda and colleagues found that knock down of the shade gene in fat body delayed pupation whereas knockdown of the shade gene in other organs had no significant effect, confirming a role of fat body in pupation timing. “We think that the fat body may incorporate the nutritional status of the animal and sends a cue for the final decision of pupation,”say Ueda and colleagues in their report.

As for other influencers of pupation timing, the orphan nuclear receptor βFTZ-F1 - to which is attached another protein factor Blimp-1 - is known to be expressed after the 20E pulse. The researchers showed that over- and underexpression of βFTZ-F1 advanced and delayed pupation respectively. They also confirmed Blimp-1’s role as a βFTZ-F1 repressor, showing that overexpression of Blimp-1 delayed pupation.

Blimp-1 is soon degraded – a protein conserved in mammals too. Studies with a line of Blimp-1 modified to enhance its stability led to delayed pupation and of the reduction of Blimp-1 expression led to advanced pupation. The researchers describe the naturally occurring Blimp-1 as like “sands in an hourglass”. As the Blimp-1 molecules degrade and levels fall, βFTZ-F1 levels increase to initiate pupation. They also showed the advantage of repressor molecule such as Blimp-1 to use precise time measuring.

Publication and Affiliation

Kazutaka Akagi1, 3*, Moustafa Sarhan1, 4*, Abdel-Rahman Sultan1, 4*, Haruka Nishida1*, Azusa Koie1, Takumi Nakayama1 and Hitoshi Ueda1,2‡ A biological timer in the fat body comprised of Blimp-1, βFTZ-F1 and Shade regulates pupation timing in Drosophila melanogaster.2016 Development doi: 10.1242/dev.133595

  • The Graduated School of Natural Science and Technology, Okayama University, Okayama, Okayama 700-8530, Japan
  • Department of Biology, Faculty of Science, Okayama University, Okayama, Okayama 700-8530, Japan
  • Present address: Aging Homeostasis Research Project Team, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan
  • Present address: Department of Zoology, Faculty of Science, Al Azhar Universty, Assiut, Egypt

*These authors are equally contributed.
‡ corresponding author, e-mail address: hueda@cc.okayama-u.ac.jp